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One-time selective XV agar medium and preparation thereof

An agar medium and a selective technology, applied in the field of disposable selective XV agar medium and its preparation, can solve the problems of time-consuming and laborious collection of sterile defibrillated sheep blood, easy occurrence of a large number of air bubbles, and batch-to-batch difference, and the like, Achieve the effect of avoiding tedious work, saving time, and eliminating interference

Inactive Publication Date: 2009-07-15
TIANJIN JINZHANG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1. The collection of sterile defibrinated sheep blood is time-consuming and laborious; 2. During the destruction of blood cells, the degree of destruction of different sheep blood is not the same, and it is easy to cause batch-to-batch differences; 3. During the preparation process, the blood may not be shaken in time place, it will cause larger particles, and shaking is also prone to a large number of air bubbles, causing great trouble for pouring

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Put beef extract powder, tryptone, sodium chloride and yeast powder into distilled water according to the ratio in Table 1, shake well, and dissolve them completely. After the dissolution is complete, use pH test paper to roughly measure the pH value of the solution, and then use 1mol / L NaOH to adjust the pH value of the solution to 7.2. Add agar powder then, after boiling and dissolving, divide in two Erlenmeyer flasks (500ml / bottle), make medium base liquid.

[0039] Put the aliquoted medium base solution into an externally heated high-pressure steam sterilizer, and sterilize at 121°C for 15 minutes. After the sterilization is finished, turn off the gas switch of the external heating high-pressure steam sterilizer. When the pointer of the pressure gauge of the sterilizer drops to zero, turn on the fixed knob on the cover and open the cover. After the hot gas is completely dissipated, the staff wear Wear work gloves, take out the bottled medium base solution, put it i...

Embodiment 2

[0043] Put the beef extract powder, tryptone, sodium chloride and yeast powder into deionized water according to the ratio in Table 1, shake well to dissolve completely. After the dissolution is complete, use pH test paper to roughly measure the pH value of the solution, and then use 1mol / L NaOH to adjust the pH value of the solution to 7.3. Then add agar powder, boil and dissolve, then divide into Erlenmeyer flasks to make medium base solution.

[0044] Put the aliquoted medium base solution into an electric high-pressure steam sterilizer, and sterilize at 121°C for 15 minutes. After the sterilization is finished, turn off the power switch of the electric high-pressure steam sterilizer. When the pointer of the pressure gauge of the sterilizer drops to zero, turn on the fixed knob on the cover and open the cover. After the heat is completely dissipated, the staff puts on the Work gloves, take out the bottled medium base solution, put it in a 54°C constant temperature water ba...

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PUM

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Abstract

The invention discloses a disposable selective XV agar culture medium which is characterized in that the culture medium comprises the following components according to the ratios thereof: 3g-5g of beef extract powder, 10g of casein tryptone, 5g of sodium chloride, 15g of agar, 5g of dusty yeast, 15mg-20mg of hemachrome, 15mg-20mg of coenzyme, 9mg of vancocin, and 1,000ml of distilled water or de-ionized water. The culture medium of the invention dispenses with the collection process of sterile defibrinated sheep blood. In the preparation process, well-prepared X and V factors are directly added before pouring, thereby avoiding complex operation of manual shaking in the corpuscle rupture process, greatly reducing preparing time and improving production efficiency.

Description

technical field [0001] The invention relates to a microbial culture medium, in particular to a disposable selective XV agar medium and a preparation method thereof. Background technique [0002] The medium commonly used in the past for the isolation and cultivation of Haemophilus is chocolate agar medium, and its preparation method is: add defibrated sheep blood aseptically to the basic medium at about 80-90°C, so that the hemocytes burst and release X factor (hemin) and factor V (coenzyme 1) for the growth of Haemophilus. Haemophilus in this medium grows well, but there are following deficiencies in its preparation process: [0003] 1. The collection of sterile defibrinated sheep blood is time-consuming and laborious; 2. During the destruction of blood cells, the degree of destruction of different sheep blood is not the same, and it is easy to cause batch-to-batch differences; 3. During the preparation process, the blood may not be shaken in time Larger particles will be ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/21
Inventor 李立津
Owner TIANJIN JINZHANG TECH DEV
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