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Method for degrading fumonisins

A fumonisin and sample technology, applied in the field of fumonisin degradation, can solve the problems affecting the reputation of the international market of agricultural products, the loss of agricultural product import and export trade, etc., and achieve the effect of obvious effect and simple operation

Inactive Publication Date: 2009-07-22
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the existence of mycotoxins has become a major obstacle in the international trade of agricultural products, causing huge losses in the import and export of agricultural products in my country and affecting the reputation of the international market for agricultural products in my country.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, reduce the fumonisin B in the contaminated corn 1 Methods

[0015] 1. Reagents used

[0016] Chromatographically pure methanol; chromatographically pure n-hexane; analytically pure sodium chloride; analytically pure formic acid; ultrapure water.

[0017] Streptococcus moniliforme (purchased from ATCC, CAS NO.52539)

[0018] Preparation of fumonisin B1 standard stock solution: prepare fumonisin B1 standard stock solution with methanol to make 40 mg / L standard stock solution, and store in the dark at 4°C.

[0019] Preparation of fumonisin B1 standard working solution: Dilute the standard stock solution with a mixture of methanol and water (the volume ratio of methanol to water is 50:50) to prepare 0.01, 0.05, 0.1, 0.5, 1, 5, 10mg / L fumonisin B1 standard working solution.

[0020] 2. Instruments used

[0021] Agilent1100 liquid phase system (Agilent, USA);

[0022] API3000 triple quadrupole mass spectrometry system (Applied Biosystems, USA);

[0023] ...

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PUM

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Abstract

The invention discloses a method for degrading fumonisins, which uses gamma rays to radiate a sample containing the fumonisins to obtain the sample with reduced fumonisins content. The method can directly radiate finished products such as agricultural products, food, and feed, has simple operation and obvious effects, and makes the degradation rate of fumonisins B1 reach 63.47 percent.

Description

technical field [0001] The invention relates to a method for degrading fumonisins. Background technique [0002] Fumonisin (F) is a group of water-soluble metabolites produced by Fusarium monliforme Sheld discovered in the late 1980s. It is one of the most harmful mycotoxins to humans and animals. Fumonisin is a structurally similar double lipid compound (FB shown in formula I) composed of different polyhydric alcohols and tricarboxylic acid 1 , FB 2 , FB 3 or FB 4 ), where fumonisin B 1 (FB 1 , C 34 h 59 O 15 N, molecular weight 721) is the major component of fumonisins in contaminating maize or Fusarium moniliforme cultures and is the main cause of the toxic effects of fumonisins (Grelderblom W.C., Jaskiewicz K., Marasas W.F., et al. Fumonisins -novel mycotoxins with cancer promoting activity produced by Fusarium moniliforme. Appl Environ Microbio, 1988, 54(7): 1806-1811). [0003] (Formula I) [0004] Corn is an important global feed resource and starch resou...

Claims

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Application Information

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IPC IPC(8): A23L1/015A23L1/025A23L5/20
Inventor 王锋哈益明周洪杰张庆芳李庆鹏王志东
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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