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Fowlpox virus rFPV-AIH5/IL2, construction method and use thereof

A fowlpox virus and IL2 technology, applied in the direction of virus/bacteriophage, antiviral agents, and microbial-based methods, can solve the problem of low immune efficacy and achieve the effect of improving immune protection and preventing H5 subtype avian influenza

Inactive Publication Date: 2009-07-22
YANGZHOU UNIV
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Problems solved by technology

[0010] The first purpose of the present invention is to provide a recombinant fowlpox virus that can be used in commercial chicken flocks that are positive for maternal antibodies in order to overcome the shortcomings of the low immune efficacy of the existing H5 subtype avian influenza recombinant fowlpox virus genetic engineering vaccine genetically engineered vaccine

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  • Fowlpox virus rFPV-AIH5/IL2, construction method and use thereof
  • Fowlpox virus rFPV-AIH5/IL2, construction method and use thereof
  • Fowlpox virus rFPV-AIH5/IL2, construction method and use thereof

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Embodiment Construction

[0056] The following relates to biological material recombinant fowlpox virus (Fowlpox virus) rFPV-AIH5 / IL2, which was deposited in the General Microbiology Center of China Committee for the Collection of Microorganisms on December 25, 2008, and the preservation number is CGMCC: 2831.

[0057] T-HA: Jia Lijun, Peng Daxin, Zhang Yanmei, et al. Construction of H5 subtype avian influenza recombinant fowlpox virus live vector vaccine and its genetic stability and immune efficacy. Acta Microbiology, 2003 43, (6): 722-727.

[0058] PT-IL2: Shao Weixing, Peng Daxin, Lu Jianhong, et al. Construction of recombinant fowlpox virus expressing chicken interleukin 2 and detection of biological activity of its expression product in vitro. Acta Biological Engineering, 2004, 20(1): 136-139.

[0059] pGEM-T easy vector: purchased from Promega.

[0060] PUC18PELNA: Chen Sujuan. Construction of single or double expression recombinant vaccines against H5 and H9 subtype avian influenza with differe...

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Abstract

The invention relates to a recombinant fowlpox virus, in particular to a recombinant fowlpox virus which co-expresses a major protective antigen gene HA of an H5 subtype avian influenza virus and a chicken interleukin 2 gene as well as a construction method and applications of the recombinant fowlpox virus. The recombinant fowlpox virus rFPV-AIH5 / IL2 co-expresses the HA gene of the H5 subtype avian influenza virus and the chicken interleukin 2 gene and has CGMCC NO. of 2831. The fowlpox virus rFPV-AIH5 / IL2 can be applied into the preparation of H5 subtype avian influenza vaccine, and compared with recombinant fowlpox virus vaccines which expresses HA gene singly, the obtained vaccine can remarkably improve the immune protection level and lower the toxin expelling level of the H5 subtype avian influenza virus after infecting chickens.

Description

technical field [0001] The invention relates to a recombinant fowlpox virus, in particular to a recombined fowlpox virus co-expressing H5 subtype avian influenza virus main protective antigen gene HA and chicken interleukin 2 gene, as well as its construction method and application. Compared with the recombinant fowlpox virus vaccine expressing HA alone, the fowlpox virus of the present invention can significantly improve the level of immune protection when used as a vaccine, and reduce the shedding level of chickens infected with H5 subtype avian influenza virus. Background technique [0002] Fowlpox Virus (FPV) is a member of the family Poxviridae and the genus Fowlpoxvirus, and is the largest animal virus ever discovered. The most unique property is that they replicate in the cytoplasm of infected cells and not in the nucleus. Mature virions are brick-shaped with a size of 250×350nm. The genome of FPV is a double-stranded linear DNA with a size of about 300kb and a molec...

Claims

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Application Information

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IPC IPC(8): C12N7/01A61K39/275A61P31/20C12R1/93
Inventor 彭大新刘秀梵云水丽陈素娟吴艳涛张小荣刘武杰
Owner YANGZHOU UNIV
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