Method for assistantly screening anti-stripe rust wheat, and special primer therefor
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A stripe rust and wheat technology, applied in the field of auxiliary screening of stripe rust-resistant wheat, can solve the problems of rust-resistant varieties losing disease resistance, accelerating the directional selection of pathogenic bacteria races, etc.
Inactive Publication Date: 2009-07-22
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology
Seedling disease resistance genes are favored by breeders due to their high resistance to stripe rust, but when planting a variety with a single disease resistance gene in a large area, it will accelerate the directional selection of pathogen races and nurture the growth of dominant race populations , causing rust-resistant varieties to lose their disease resistance after a few years of large-scale application in production
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Embodiment 1
[0024] Example 1, Discovery of Xrga-1 and rgga-1
[0025] Zhou 8425B showed moderate resistance (IT=2) to high resistance (IT=1) to Tiaozhong 32 at the seedling stage, and high resistance (IT=1) at the adult stage. Both the spring seedling stage and adult plant stage in China were highly sensitive to Tiaozhong 32 (IT=4).
[0026] F 1 group, F 1 The population is 35 individual plants. Will F 1 F 2 group. f 2 The population is 522 individual plants.
[0027] 1. Acquisition of special primer pairs
[0028] in F 2 In a single plant, 20 seedling reaction types were selected as 2, and the 7 SSR marker genotypes linked to the disease-resistant gene on the 7BL chromosome were all mixed with the DNA of a single plant consistent with the disease-resistant parent Zhou 8425B in equal amounts, as an anti- pool. in F 2From a single plant, 20 seedling stage response types were selected as 4, and the 7 SSR marker genotypes on the 7BL were all mixed with the DNA of a single plant c...
Embodiment 2
[0046] The application of embodiment 2, Xrga-1 and rga-1
[0047] Zhoumai 11, Zhoumai 12, Zhoumai 13, Zhoumai 16, Zhoumai 17, Zhoumai 22, Aikang 58, 04-zhong 36, Fu 98-46 and Luomai 22 are descendants of Zhou 8425B (Zhuang Qiaosheng Editor-in-Chief, Chinese Wheat Variety Improvement and Pedigree Analysis, Beijing / China Agricultural Press, 2003.1).
[0048] 1. Detect whether different varieties of wheat carry rga-1
[0049] The genomic DNA of each variety of wheat was extracted, and the genomic DNA was used as a template to carry out PCR amplification with Xrga-1 respectively.
[0050] The 15 μl PCR reaction system is: 20ng template DNA, 150 μM dNTPs (TaKaRa Company), 0.25U Taq DNA polymerase (Promega Company), 7 pmol of upstream and downstream primers, 1.5 μl 10×PCR buffer (Promega Company), 1.5 mM MgCl 2 (Promega Corporation).
[0051] The PCR reaction was carried out on a PTC-200 PCR amplification instrument. The amplification program was: pre-denaturation at 95°C for 5 ...
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Abstract
The invention discloses a filtration assisting method of yellow rust resistant wheat varieties and a specific primer. A filtration assisting primer pair of yellow rust resistant wheat varieties that is provided by the invention consists of a DNA that is shown in sequence 1 of the sequence table and a DNA that is shown in sequence 2 of the sequence table. The filtration assisting method of yellow rust resistant wheat varieties that is provided by the invention comprises the following steps: a genomic DNA of a wheat variety to be detected is used as a template, the primer pair is used for PCR augmentation, an augmented product is detected, and the wheat variety to be detected with a 343bp strip in the augmented product is yellow rust resistant wheat candidate. The invention further provides a molecular marker of a yellow rust resistance gene YrZH8 that is a DNA shown in sequence 3 of the sequence table. The molecular marker has closer linkage with YrZH84 and consequently has high efficiency in the breeding of yellow rust resistant wheat molecules and the cloning of the yellow rust resistance gene YrZH84. The primer pair and the molecular marker of the invention have important actions in the breeding of yellow rust resistant wheat varieties.
Description
technical field [0001] The invention relates to a method for assisting in screening wheat resistant to stripe rust and special primers thereof. Background technique [0002] Stripe rust caused by wheat stripe rust (Puccinia striiformis f.sp.tritici) is a widely distributed airborne disease that occurs in almost all major wheat producing countries, especially in cold and high altitude regions. my country is the largest endemic area of wheat stripe rust in the world, and wheat stripe rust mainly occurs in the northwest and southwest regions of China. From the 1950s to the present, there have been 15 moderate epidemics and 7 large-scale wheat stripe rust epidemics in my country. The area of the first occurrence of wheat stripe rust reached more than 200 million mu, and the area of the last two outbreaks was more than 110 million mu, with a loss of 600, 3 million, 2.65 and 1 million tons of wheat respectively (Wan Anmin et al., Review of Wheat Stripe Rust Occurrence in my ...
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