Method for reinforcing salt endurance of vegetable crop

A technology of salt tolerance and vegetables, applied in the field of soil microorganisms, to reduce ethylene content, increase fresh weight and antioxidant enzyme activity, and ensure the effect of growth

Inactive Publication Date: 2009-07-29
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has been no report on the use of Pseudomonas and plants to construct a "plant-microbe" system to improve the growth of vegetable crops in coastal saline land

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027]Embodiment 1: (screening with 1-aminocyclopropane 1-carboxylate deaminase activity strain)

[0028] Follow these steps:

[0029] (1) Enrichment culture of bacterial strains: Suspend the Zhejiang Shangyu tidal flat soil sample in PAF medium (each L contains 10g peptone, 10g casein hydrolyzate, 1.5g MgSO 4 , 1.5g K 2 HPO 4 ), shaker at 28°C-35°C 200r·min -1 Shake culture for 24 hours, take the suspension and transfer it to DF medium (each L contains 4.0gKH 2 PO 4 , 6.0 g Na 2 HPO 4 , 0.2g MgSO 4 ·7H 2 O, 2.0g glucose, 2.0g gluconic acid, 2.0g citric acid, 2.0g (NH 4 ) 2 SO 4 , pH7.2-7.4), cultivated under the same conditions for 24 hours, to obtain the enriched culture solution;

[0030] (2) Culture medium formula and strain isolation and purification: Take the enriched culture medium and transfer it to DFa medium (ACC is dissolved in ultrapure water and then sterilized by suction) with 1-aminocyclopropane 1-carboxylic acid as the only nitrogen source , added ...

Embodiment 2

[0033] Embodiment 2: (identification of bacterial strain DW1 with 1-aminocyclopropane 1-carboxylate deaminase activity)

[0034] The bacterial strain DW1 screened in Example 1 was identified according to "Bergey's Bacteria Identification Manual (Eighth Edition)" and "Common Bacteria System Identification Manual", and the total DNA was extracted according to molecular cloning three, using primers: BSF8 / 20, 5'-AGAGTTTGATCCTGGCTCAG-3' (Escherichia coli corresponds to position 8-27); reverse primer BSR1541 / 20, 5'-AAGGAGGTGATCCAGCCGCA-3' (E. coli corresponds to position 1541-1522) to amplify the strain The 16S rDNA gene of the strain was entrusted to Invitrogen to amplify and sequence the 16S rDNA of the strain. After obtaining the 16S rDNA sequence of the strain, the 16S rRNA gene sequence of the related strain in GenBank was searched by BLAST on the NCBI website, and the homology comparison was carried out. right. Based on the identification of morphology, physiological and bioc...

Embodiment 3

[0035] Embodiment 3: (screening of salt-tolerant vegetable varieties under salt stress conditions)

[0036] Follow these steps:

[0037] (1) Seed germination experiment: Carry out NaCl concentration in the 9cm petri dish of diameter respectively: 0 (CK), 0.1%, 0.2%, 0.3% seed germination culture test, all concentrations are all established 3 times to repeat; Each petri dish Sow 30 seeds, add 10ml of a certain concentration of NaCl solution once in each treatment, cultivate at 28°C, record the number of germinated seeds on the 7th day of germination and calculate the germination rate; germination rate (%)=number of germinated seeds on the 7th day / Sow the number of seeds × 100%, measure the bud length at the same time;

[0038] (2) Pot experiment: select the kind with high seed germination rate to further carry out pot experiment. The test bowl is a plastic bowl with a height of 12cm and a diameter of 10cm. Each bowl is filled with 0.5kg of soil (Hangzhou Bay coastal saline so...

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Abstract

The invention relates to a method for enhancing the salt endurance of vegetable crops and belongs to the technical field of edaphon. In the invention, a pseudomonad strain DW1 CGTCC No.2729 with high 1-amino-cyclopropane 1-carboxylic acid deaminase activity is first screened and obtained from coastal soils; further more, a method for enhancing the salt endurance of vegetable crops is proposed; the method comprises the steps as follows: the preparation of a DF culture medium; the activating culture of the strains; the fermentation culture of the strains; the screening of salt-resistant vegetable variety, and the construction of vegetable variety-microbic system. The method can remarkably improve the fresh weight of the vegetable crops under a salt concentration of 0.1 percent and 0.2 percent as well as the content of Caand k ions, reduce the content of proline and Na ion, simultaneously increase the antioxidase activity of seedlings, conduce to the plant growth under a salt stress condition and improve the productivity. The invention can be popularized and applied in improving and using seashore saline soil.

Description

technical field [0001] The invention relates to the technical field of soil microbes, in particular to isolated and screened bacterial strains with 1-aminocyclopropane 1-carboxylic acid (ACC) deaminase activity and vegetable varieties with better salt tolerance, through the construction of " Plant-microbe" joint approach to achieve a method to alleviate the damage of seashore soil salinity to vegetable crops. Background technique [0002] Agriculture is the primary industry on which human beings depend for survival. With the growth of population, the demand for agriculture is also increasing, and the salinization of soil restricts the development of agriculture and becomes a worldwide resource and ecological problem. According to statistics, 20% of the world's cultivated land and nearly half of the irrigated land are subject to varying degrees of salt stress. As far as our country is concerned, there are more than 1×10 9 hm 2 All kinds of saline soil, among which modern ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01H1/04A01C1/06A01G7/06C12R1/38
Inventor 刘琛傅庆林丁能飞林义成郭彬
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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