Biological preparation containing plant growth promoting bacteria, as well as preparation and use thereof

A technology of plant growth-promoting bacteria and biological agents, which is applied in the direction of organic fertilizers, can solve the problems of limiting the reproduction opportunities of other pathogenic microorganisms, and achieve the effects of improving crop nutritional conditions, increasing yield, and good solubility

Inactive Publication Date: 2009-08-05
黄晓东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3. Biological control of harmful microorganisms. Due to the inoculation of microbial fertilizers at the roots of crops, microorganisms grow and reproduce in large

Method used

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  • Biological preparation containing plant growth promoting bacteria, as well as preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0137] Embodiment 1. Composition of the biological agent of the present invention:

[0138] Composite carrier material: 25% peat (organic matter), 22% humic acid, 22% decomposed organic matter, 12% complex replacement agent (enzymolysis (digestion) protein 8% + calcium sulfate 4%), 0.05% of methyl cellulose and the rest of the granulating agent.

[0139] Bacterial solution: the bacterial solution containing UW3 and UW4 with a bacterial ratio of 1:1, concentration: 200 million bacterial counts / 0.05ml.

[0140] Preparation:

[0141] 1. First carry out industrial fermentation of the strains of UW3 and UW4 to make fermentation broth, the method is as follows:

[0142] (1). Take 1 gram of plant rhizosphere soil, immerse in 50ml of PAF extraction solution, and then put it into a 250ml container; 4 、K 2 HPO 4 and a solution of the composition of glycerol;

[0143] (2). Shake the above-mentioned conical flask containing 50ml of PAF extraction solution at 25±2°C;

[0144] (3). G...

Embodiment 2

[0162] Embodiment 2. Composition of the biological preparation of the present invention:

[0163] Composite carrier material: 27% peat (organic matter), 20% humic acid, 20% decomposed organic matter, 13% complex replacement agent (including enzymatic (digested) protein 10% + calcium sulfate 3%), 0.05 % of methyl cellulose, the rest of the granulating agent;

[0164] Bacteria solution: The bacteria solution containing 2:1 (bacterial number ratio) of UW3 and UW4, the concentration is 0.01ml to reach 200 million bacteria.

[0165] Preparation:

[0166] Referring to Example 1, the soil for preparing the fermentation broth is different from the plant rhizosphere soil in Example 1, and the plant rhizosphere soil in a region where the latitude and longitude of the geographic location of the soil in Example 1 is very different.

[0167] According to the analysis and identification of 16s rRNA gene program, the strains contained in the fermentation broth and biological preparation ob...

Embodiment 3

[0168] Example 3. Composition of the biological preparation of the present invention:

[0169] Composite carrier material: 22% peat (organic matter), 22% humic acid, 22% decomposed organic matter, 16% complex replacement agent (including enzymatic (digested) protein 11% + calcium sulfate 5%), 0.05 % of methyl cellulose, the rest of the granulating agent;

[0170] Bacteria solution: The bacteria solution containing UW3 and UW4 at a ratio of 1:2 (number of bacteria), the concentration is 0.1ml to reach 200 million bacteria.

[0171] Preparation:

[0172] With reference to Example 1, the soil for preparing the fermentation broth is different from the plant rhizosphere soil in Examples 1 and 2, and the plant rhizosphere soil in a region where the latitude and longitude of the geographical location of the soil in Examples 1 and 2 differ greatly.

[0173] According to the analysis and identification of 16s rRNA gene program, the strains contained in the fermentation broth and biol...

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Abstract

The invention provides a plant growth-promoting bacterium, a biological agent containing the plant growth-promoting bacterium and a method for preparing the bacterium and the biological agent. The biological agent contains a composite carrier material and a bacterial liquid, wherein the composite carrier material comprises an organic matter, humic acid, a decomposed organic matter, a complex displacer and an embedding material; the bacterial liquid is a solution which contains the plant growth-promoting bacterium and comprises UW3 and UW4 with a proportion of bacteria number being 1-2:2-1; and the composite carrier material and the bacterial liquid lead the bacteria number of the plant growth-promoting bacterium to reach at least 0.1 billion/per gram composite carrier material so as to obtain the biological agent. The biological agent used in agricultural production can promote plant growth, improve yield, and improve manurial effect and anti-adversity capability of the plant.

Description

technical field [0001] The present invention relates to a microbial fertilizer, in particular to a plant growth-promoting bacterium and a biological preparation (microbial preparation), especially a biological preparation containing multifunctional plant growth-promoting bacterium components. Background technique [0002] Microbial fertilizer refers to a specific preparation containing living microorganisms, which can obtain specific fertilizer effects when applied in agricultural production. Microbial fertilizers can be divided into two categories. One is to increase the supply of plant nutrients through the life activities of microorganisms contained in it, improve the nutritional status of plants, and then increase production. The representative variety is bacterial fertilizer; the other is generalized fertilizer. Microbial fertilizers, although their products also increase crop production through the microbial life activities contained in them, but it is not limited to i...

Claims

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Application Information

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IPC IPC(8): C05F11/08C05F11/02
Inventor 黄晓东
Owner 黄晓东
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