Biological preparation containing plant growth promoting bacteria, as well as preparation and use thereof
A technology of plant growth-promoting bacteria and biological agents, which is applied in the direction of organic fertilizers, can solve the problems of limiting the reproduction opportunities of other pathogenic microorganisms, and achieve the effects of improving crop nutritional conditions, increasing yield, and good solubility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0137] Embodiment 1. Composition of the biological agent of the present invention:
[0138] Composite carrier material: 25% peat (organic matter), 22% humic acid, 22% decomposed organic matter, 12% complex replacement agent (enzymolysis (digestion) protein 8% + calcium sulfate 4%), 0.05% of methyl cellulose and the rest of the granulating agent.
[0139] Bacterial solution: the bacterial solution containing UW3 and UW4 with a bacterial ratio of 1:1, concentration: 200 million bacterial counts / 0.05ml.
[0140] Preparation:
[0141] 1. First carry out industrial fermentation of the strains of UW3 and UW4 to make fermentation broth, the method is as follows:
[0142] (1). Take 1 gram of plant rhizosphere soil, immerse in 50ml of PAF extraction solution, and then put it into a 250ml container; 4 、K 2 HPO 4 and a solution of the composition of glycerol;
[0143] (2). Shake the above-mentioned conical flask containing 50ml of PAF extraction solution at 25±2°C;
[0144] (3). G...
Embodiment 2
[0162] Embodiment 2. Composition of the biological preparation of the present invention:
[0163] Composite carrier material: 27% peat (organic matter), 20% humic acid, 20% decomposed organic matter, 13% complex replacement agent (including enzymatic (digested) protein 10% + calcium sulfate 3%), 0.05 % of methyl cellulose, the rest of the granulating agent;
[0164] Bacteria solution: The bacteria solution containing 2:1 (bacterial number ratio) of UW3 and UW4, the concentration is 0.01ml to reach 200 million bacteria.
[0165] Preparation:
[0166] Referring to Example 1, the soil for preparing the fermentation broth is different from the plant rhizosphere soil in Example 1, and the plant rhizosphere soil in a region where the latitude and longitude of the geographic location of the soil in Example 1 is very different.
[0167] According to the analysis and identification of 16s rRNA gene program, the strains contained in the fermentation broth and biological preparation ob...
Embodiment 3
[0168] Example 3. Composition of the biological preparation of the present invention:
[0169] Composite carrier material: 22% peat (organic matter), 22% humic acid, 22% decomposed organic matter, 16% complex replacement agent (including enzymatic (digested) protein 11% + calcium sulfate 5%), 0.05 % of methyl cellulose, the rest of the granulating agent;
[0170] Bacteria solution: The bacteria solution containing UW3 and UW4 at a ratio of 1:2 (number of bacteria), the concentration is 0.1ml to reach 200 million bacteria.
[0171] Preparation:
[0172] With reference to Example 1, the soil for preparing the fermentation broth is different from the plant rhizosphere soil in Examples 1 and 2, and the plant rhizosphere soil in a region where the latitude and longitude of the geographical location of the soil in Examples 1 and 2 differ greatly.
[0173] According to the analysis and identification of 16s rRNA gene program, the strains contained in the fermentation broth and biol...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com