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Method for rapidly identifying cysteine and cystine by utilizing electrochemical technique

A chemical technology, cysteine ​​technology, is applied in the field of rapid identification of cysteine ​​and cystine by using electrochemical technology, and the field of identification of cysteine ​​and cystine, which can solve the problem of expensive equipment purchase and maintenance costs, no It is beneficial to problems such as batch type, rapid determination, and cumbersome detection process, and achieves the effects of convenient maintenance, low price, and simple process

Inactive Publication Date: 2009-08-19
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the derivatization process makes the detection process cumbersome and brings a large number of new environmental pollutants
At the same time, the equipment purchase and maintenance costs required by the above identification techniques are relatively expensive, which is not conducive to the realization of batch and rapid determination.

Method used

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  • Method for rapidly identifying cysteine and cystine by utilizing electrochemical technique

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Phosphate electrolyte with a concentration of 0.01-0.2 mol / L is prepared by mixing equal concentrations of phosphoric acid and disodium hydrogen phosphate in different proportions, and adjusting its pH value to 4.5-8.5 with a pH meter.

[0020] Preferably, the concentration of the phosphate electrolyte is 0.1-0.2 mol / L, and the pH range is 5.5-7.5.

[0021] The three-electrode system uses a polycrystalline gold disk electrode (CHI101) as the working electrode, a saturated calomel electrode as the reference electrode, and a platinum wire electrode as the counter electrode. Before the application of the working electrode, use metallographic sandpaper, 1um, 0.3um or 0.05umAl 2 o 3 The powder was polished to a mirror surface, soaked in Piranha solution for 10 minutes and ultrasonically cleaned with absolute ethanol before use.

[0022] Take the sample to be tested containing cysteine ​​and cystine and dissolve it in the above-mentioned phosphate electrolyte to prepare a s...

Embodiment 2

[0024] Mix equal concentrations of phosphoric acid and disodium hydrogen phosphate in proportion to prepare a phosphate electrolyte with a concentration of 0.1 mol / L, and adjust its pH value to 6.25 with an acidity meter.

[0025] The three-electrode system uses a polycrystalline gold disk electrode (CHI101) as the working electrode, a saturated calomel electrode as the reference electrode, and a platinum wire electrode as the counter electrode. Before the application of the working electrode, use metallographic sandpaper, 1um, 0.3um or 0.05umAl 2 o 3 The powder was polished to a mirror surface, soaked in Piranha solution for 10 minutes and ultrasonically cleaned with absolute ethanol before use.

[0026] Take analytically pure cysteine ​​and cystine and dissolve them in 0.1mol / L phosphate electrolyte (pH=6.25) to prepare a sample solution containing 2mmol / L. , to scan potential -2V ~ 2V, scan mode: negative scan, scan rate: 100mV / min, scan interval: 0.05s, response time: 0....

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Abstract

The invention discloses a new method for rapidly distinguishing cysteine and cystine by using an electrochemical technology. The method adopts a three-electrode system which takes a polycrystalline gold disc electrode as a working electrode, a saturated calomel electrode as a reference electrode, and a platinum wire electrode as a counter electrode, and realizes the rapid distinguishing of the compounds by scanning cysteine and cystine solution in a cyclic voltammetry way under the condition of phosphate medium. The method has the advantages that the operation is simple and convenient, and the method is economical and applicable, clean without secondary pollution, and capable of continuous determination for a plurality of times, and the like.

Description

technical field [0001] The invention relates to a method for discriminating cysteine ​​and cystine, in particular to a new method for quickly discriminating cysteine ​​and cystine by using electrochemical technology, and belongs to the technical field of environment and chemistry. Background technique [0002] Cysteine ​​is the simplest sulfur-containing compound in organisms and plays an important role in disease prevention and regulation. As the main antioxidant, cysteine ​​can effectively remove excess oxidative active substances produced in the metabolic process, avoid the oxidative damage of other functional biomolecules, and oxidize itself into cystine. Under normal physiological conditions, the cysteine / cystine content of the body can be maintained at a certain level, but diseases and aging processes promote the consumption of cysteine ​​and the accumulation of cystine, causing changes in the content of both. Therefore, the change of cysteine / cystine can be used to m...

Claims

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Application Information

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IPC IPC(8): G01N27/48C07C323/58
Inventor 刘汝涛宗万松孙凤
Owner SHANDONG UNIV
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