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Methods for making and using wheat plants with increased grain protein content

A protein and high-protein technology, applied in the field of agriculture, can solve problems such as complex environmental interactions and slow progress

Inactive Publication Date: 2009-09-02
BASF AGROCHEMICAL PROD BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although much effort has been put into breeding for high grain protein content, progress has been slow due to the complexity of the genetics controlling the trait and its interaction with the environment

Method used

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  • Methods for making and using wheat plants with increased grain protein content
  • Methods for making and using wheat plants with increased grain protein content
  • Methods for making and using wheat plants with increased grain protein content

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preparation example Construction

[0006] The present invention provides methods of making and using wheat plants comprising grain with increased grain protein content. The present invention comprises introducing into a wheat plant at least one copy of the wheat AHASL1A gene encoding an AHASL1A protein comprising the S653N substitution. Such genes can be introduced by methods such as cross-pollination, mutagenesis and transformation. The methods of the invention may further comprise growing a wheat plant comprising the AHASL1A S653N gene, or a progeny thereof, to produce grain, and determining the protein content of the grain produced by said wheat plant or progeny. Wheat plants produced by the methods of the invention and progeny plants thereof comprise increased grain protein content when compared to wheat plants not containing the wheat AHASL1A S653N gene.

[0007] The methods of the invention can be used to develop new wheat cultivars with increased grain protein content. When compared with existing metho...

Embodiment 1

[0135] Example 1: Wheat lines with increased grain protein content

[0136] Wheat lines were generated using standard mutagenesis and conventional plant breeding methods. The purpose of mutagenesis is to develop wheat lines that are resistant to imidazolinone herbicides. The mutation responsible for imidazolinone herbicide resistance in these wheat lines was a single nucleotide change from guanine to adenine, which resulted in a codon change from AGC to AAC and in AHASL (acetohydroxyacid synthase large sub base) protein from a single amino acid substitution from serine to asparagine, which was designated TaAHASL1A S653N. The AHAS enzyme catalyzes the first step in the biosynthesis of branched-chain amino acids (valine, leucine, and isoleucine) (Stidham and Singh (1991) "Imidazolinone-Acetohydroxyacid Synthase Interactions," The Imidazolinone Herbicides, Ch.6, Shaner, D., and O'Connor, S., eds.; CRC Press, Boca Raton, Florida, U.S.A., pp. 71-90), and is subject to feedback re...

Embodiment 2

[0154] Example 2: Herbicide-resistant wheat AHASL protein

[0155]The invention discloses the application of the polynucleotide encoding the wheat AHASL1A S653N polypeptide. Plants comprising herbicide-resistant AHASL polypeptides have been previously identified, and a number of conserved regions of AHASL polypeptides, the sites of amino acid substitutions that confer herbicide resistance, have been described. See Devine and Eberlein (1997) "Physiological, biochemical and molecular aspects of herbicide resistance based on altered target sites", Herbicide Activity: Toxicology, Biochemistry and Molecular Biology, Roe et al. (eds.), pp.159-185, IOS Press, Amsterdam; and Devine and Shukla, (2000) Crop Protection 19:881-889.

[0156] Using the wheat AHASL1A S653N sequence of the invention and methods known to those skilled in the art, additional polynucleotides encoding herbicide-resistant AHASL polypeptides having the S653N substitution and the identified AHASL polypeptides in th...

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Abstract

The present invention provides novel methods for making wheat plants with increased grain protein content. The methods involve introducing a gene encoding herbicide-resistant, wheat acetohydroxyacid synthase large subunit (AHASL) protein. The invention further provides wheat plants that produce high protein grain and human and animal food products derived thereof.

Description

field of invention [0001] The present invention relates to the field of agriculture and in particular to novel methods for preparing and using wheat plants with increased grain protein content. Background technique [0002] The grain protein content of wheat is important for improved nutritional value and is a major contributor to bread making (Dick & Youngs (1988) "Evaluation of durum wheat, semolina, and pasta in the United States", Durum Wheat: Chemistry and technology, AACC, St. Paul, MN, pp.237-248; Finney et al. (1987) "Quality of hard, soft, and durum wheats". E.G. Heyne (ed.) Wheat and wheat improvement, Agron. Monogr. 13, 2nd Edition, ASA, CSSA, and SSSA, Madison, WI, pp. 677-748; Khan et al. (2000) Crop Sci. 40: 518-524). It is also an important trait for growers due to the premium of wheat with high grain protein (Olmos et al. (2003) Theor. Appl. Genet. 107:1243-1251). Although much effort has been devoted to breeding for high grain protein content, progress has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/10A23L7/10
CPCC12N15/8251A01H5/10A01H6/4678
Inventor W·J·霍维R·E·凯勒D·R·卡尔森M·L·达莫尔B·K·辛
Owner BASF AGROCHEMICAL PROD BV