Susceptible/resistant MUC13 molecular marker capable of identifying F4 weaning brash of piglets and application

A technology of molecular markers and weaned piglets, which is applied in the field of animal molecular biology technology and breeding, can solve problems that affect human health, easily cause drug resistance, and increase feeding costs

Inactive Publication Date: 2009-09-16
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Escherichia coli disease seriously affects the growth and development of piglets, leading to increased feeding costs and huge economic losses to the pig industry. Vaccination or antibacterial drugs are used to prevent and treat the disease. On the one hand, long-term use is likely to cause drug resistance. The abuse of drugs will lead to drug residues in animal products and become drugs and food, which will eventually affect human health

Method used

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  • Susceptible/resistant MUC13 molecular marker capable of identifying F4 weaning brash of piglets and application
  • Susceptible/resistant MUC13 molecular marker capable of identifying F4 weaning brash of piglets and application
  • Susceptible/resistant MUC13 molecular marker capable of identifying F4 weaning brash of piglets and application

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Embodiment

[0054] 144 purebred piglets including Duroc, Landrace and Large White pigs were used as experimental animals. These pigs were purchased from 5 large-scale commercial pig farms. Each pig breed selected at least 3 male lines, and each family selected 3-4 A 1-2 month old individual. After slaughtering 144 purebred piglets, the jejunum part of the small intestine was taken, and the brush border of small intestinal epithelial cells was extracted. E.coli F4ab, F4ac and F4ad strains were used to adhere to the brush border of pig small intestinal epithelial cells, and the results of bacterial adhesion were detected by phase contrast microscopy. More than 20 brush borders were detected for each sample. If at least 2 bacteria adhered to more than 10% of the brush borders, the sample was judged as adherent type. If at least 2 bacteria adhered to the brush borders, less than 10 %, but there are more than 10% of brush borders with 1-2 bacterial adhesion, the sample is judged as weak adhesi...

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Abstract

The invention discloses a susceptible/resistant MUC 13 molecular marker capable of identifying F4 weaning brash of piglets of western pig breeds and application thereof to genetic improvement of pig breeds. The molecular marker firstly identifies single nucleotide polymorphism (SNP) A157G, and then adopts a SNaPshot method to perform genotype judgment on the SNPA157G. The invention detects a polymorphic site through modern molecular biology technology, utilizes the marker assisted selection (MAS) to select advantageous allelotype reserved individual breed, can obviously improve weaning brash resistance of species groups, and greatly reduces the death rate of the piglets.

Description

technical field [0001] The invention relates to the field of animal molecular biology technology and breeding, in particular to a MUC13 molecular marker capable of identifying the susceptibility / resistance to weaned piglet F4 diarrhea and its application. Background technique [0002] Infectious diseases of livestock and poultry cause huge economic losses to livestock and poultry production all over the world every year. The losses caused by this account for 17% (1.7 billion pounds) of the annual trade volume in developed countries, and as high as 35%-50% in developing countries. Piglet diarrhea is the most common and very important disease. The mortality rate of piglets caused by diarrhea is 11.5%-29.5%. Among them, the morbidity and mortality caused by E. coli account for 56.2% of the incidence and mortality of pig diarrhea and 24.7%. Escherichia coli disease seriously affects the growth and development of piglets, leading to increased feeding costs and huge economic loss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q2600/156C12Q1/6883
Inventor 黄路生任军晏学明黄翔张志燕欧阳婧曾维宏
Owner JIANGXI AGRICULTURAL UNIVERSITY
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