Powder for chicken sperm dilution and preservation and preparation method and application thereof
A semen and diluent technology, applied in the field of artificial insemination of poultry, can solve the problems of long storage time, hectic transportation of chicken semen, and unsolved problems of chicken semen preservation, achieve low cost, enrich research results, and improve immediate insemination busy effect
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[0034] Example 1 According to the diluent powder formula given in Table 1, prepare the raw materials:
[0035] Table 1 Diluent powder formula
[0036] Reagent content
[0037] glucose 0.2400g Sodium citrate 0.0250g Sodium Glutamate 0.4005g K 2 HPO 4 .3H 2 O 0.62055g K 2 HPO 4 0.0325g Sodium acetate trihydrate 0.2050g NaCl 0.0900g Tris 0.0415g penicillin 0.0100g
[0038] Various chemicals (analytical grade) in the purchased formula, large particles or agglomerated ingredients need to be crushed and sieved through an 80-mesh sieve to form powder.
[0039] Accurately weigh various ingredients, mix them according to the proportions in the formula, and stir them thoroughly, then pack them into bags, evacuate and seal to obtain 1 packet of chicken semen dilution powder.
[0040] The above process generally requires the temperature to be controlled at 18 to 26°C and the relative humidity to be controlled a...
Example Embodiment
[0045] Example 2 According to the diluent powder formula given in Table 2, prepare the raw materials:
[0046] Table 2 Diluent powder formula
[0047] Reagent content glucose 0.2000g Sodium citrate 0.0150g Sodium Glutamate 0.4500g K 2 HPO 4 .3H 2 O 0.5500g K 2 HPO 4 0.0500g Sodium acetate trihydrate 0.3000g NaCl 0.0500g Tris 0.0900g Chloramphenicol 0.0100g
[0048] Other operations are the same as in Example 1. With 60ml of distilled water to completely dissolve and adjust the PH value to 8.0, the semen can be directly infused after being diluted by 1:2 in the diluent, or it can be stored at 4°C for later use.
Example Embodiment
[0049] Example 3 According to the diluent powder formula given in Table 3, prepare the raw materials:
[0050] Table 3 Diluent powder formula
[0051] Reagent content glucose 0.3000g Sodium citrate 0.0270g Sodium Glutamate 0.3500g K 2 HPO 4 .3H 2 O 0.7500g K 2 HPO 4 0.0100g Sodium acetate trihydrate 0.3000g NaCl 0.1500g Tris 0.0100g Streptomycin 0.0100g
[0052] Various chemicals (analytical grade) in the purchased formula, large particles or agglomerated ingredients need to be crushed and sieved through an 80-mesh sieve to form powder. Other operations are the same as in Example 1. The diluted semen can be insemated directly or stored at 4°C for later use.
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