3-sterone-delta[1]-dehydrogenase gene, relevant carriers, engineering strains and applications thereof

A technology of genetic engineering and dehydrogenase, applied in the field of molecular biology, 3-sterone-△1-dehydrogenase, can solve the problems of high toxicity, low conversion efficiency, steroid nucleus degradation, serious environmental pollution, etc.
CN101565710AActive Publication Date: 2009-10-28EAST CHINA UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Publication Date
2009-10-28

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Abstract

The invention provides a 3-sterone-Delta[1]-dehydrogenase. By constructing various expression vectors and converting relevant strains, the invention obtains various engineering strains such as non-3-sterone-Delta[1]-dehydrogenase activity strains or high 3-sterone-Delta[1]-dehydrogenase activity strains; the engineering strains can be adopted to selectively prepare androst-4-alkene-3, 17-diketone and 9Alpha-androst-4-alkene-3, 17-diketone or androst-1, 4-diene-3, 17-diketone and 3-ketone-1, 4-dienes steroid compounds. The engineering strains can greatly improve the production efficiency and product quality of the steroid medical production system, are beneficial for reducing the energy consumption of steroid drugs during the production process, improving the utilization ratio of the prodrugs and simplifying the production steps, reduce the production cost, have moderate reaction conditions and friendly environment, are suitable for wide generalization and application and have higher economic benefits and social benefits.
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Description

technical field

[0001] The present invention relates to the technical field of molecular biology, more specifically, relates to 3-sterone-△ 1 -Dehydrogenase technical field, especially a kind of 3-sterone-△ 1 -Dehydrogenase gene, related vectors and engineering bacteria and application. Background technique

[0002] Steroids, also known as steroids, are a class of compounds with cyclopentane polyhydrophenanthrene as the core and similar structures. Its basic structure is shown in the figure below. Due to the difference in substituents, double bond positions or stereo configurations on the steroid core, a series of compounds with unique physiological functions have been formed. In animals, steroids are the main endogenous sex hormones, secreted by sex organs and adrenal cortex, and closely related to reproduction, bone and brain development, regulation of biological effects, and maintenance of homeostasis. Steroid drugs mostly refer to steroid hormone drugs, such as adrena...

Claims

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