Petunia somatocyte cell natural mutation individual tissue culture expanding propagation method

Abstract

The invention relates to a petunia somatocyte cell natural mutation individual tissue culture expanding propagation method, which comprises the following steps of: step one: subculture expanding propagation of mutation plantlets of the petunia, the plantlets are taken as explants and cultured in a culture medium which is particularly confected and suitable for the growing of the plantlets so as to obtain new gemmules which obviously grow and split apart; step two: root induction and transplantation, the gemmules are reinoculated into the culture medium for inducing the root to be cultured for 60 days; in the normal situation, the rootage reaches 100%; the gemmules are moved to an acclimatization room to be acclimated for two weeks and then is transplanted into an artificial medium; after one week, the gemmules are moved into the room temperature to be grown. The method can overcome difficult problems of excellent character preservation of the autogamous sterility of the petunia and the difficult rooting of sticking, and quickly breeds and plants mutation petunia plantlets with natural mutation and stable genetic characteristic which are hard to find, and breeds the new petunia specimens with ornamental value.

Description

technical field [0001] The invention relates to a method for tissue culture of flower mutation individuals to expand population reproduction. Background technique [0002] Petunia (Petunia hybrida Vilm) is a perennial herbaceous plant of the genus Petunia (Petunia) in the Solanaceae family. It is often cultivated as one or two years. The plant height is 15-60cm. The whole plant is covered with sticky hairs, and the base of the stem is woody. The young stems are erect, and the old stems are prostrate. Single leaves alternate, ovate, entire, nearly sessile, upper leaves opposite. Flowers solitary in leaf axils or terminal, larger, corolla funnel-shaped, margin 5-lobed. The flowering period is from April to October. Capsules, small seeds. [0003] The applicant cultured petunia leaf tissue in August 1998 to make adventitious buds germinate directly on the leaves, then cultivated them in test tubes to take root and then transplanted them into potted plants until they bloomed...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to use the method of tissue culture breeding and breeding of the rare petunia seedlings formed by the natural mutation of this cell, to preserve the traits and expand the population, and to provide the society with a more ornamental value faster and more widely. New species of petunias, adding a new member to the petunia family