Extract of bupleurum, preparation method and application thereof

The technology of Bupleurum chinensis extract and Bupleurum chinensis is applied in the directions of drug combination, pharmaceutical formula, plant raw material, etc., can solve the problems of the preparation of traditional Chinese medicine and natural medicine for cholestatic hepatitis, etc., achieves good therapeutic effect, and has a simple preparation method. , the effect of low cost

Inactive Publication Date: 2011-10-19
THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But up to now, there is not yet the preparation of ideal Chinese medicine and natural medicine for the treatment of stasis hepatitis.

Method used

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  • Extract of bupleurum, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038](1) Dry and crush 3000 grams of stems and leaves of Bupleurum smithii Wolffvar.Pavuifolium Shan et Y.L i into about 20 meshes, add 9 times the weight of 85% ethanol and extract at 85°C for 3 times under reflux, each extraction After 3 hours, combine the extracts, filter, recover ethanol and concentrate until the relative density is 1.0-1.1;

[0039] (2) Add 9 times the volume of water to the concentrated solution, leave it for 12 hours, filter to obtain the filtrate;

[0040] (3) The filtrate obtained in step 2 is passed through a D101 type macroporous resin column, the flow rate is 5ml / min, and water is eluted, and the Molishi reaction is negative to determine the end point of water elution; 70% ethanol is eluted, and the eluent is collected, TLC Detect that rutin no longer appears to determine the end point, recover ethanol, and vacuum dry at 70° C. to obtain 600 g of Bupleurum extract powder.

Embodiment 2

[0042] (1) 3000g of stems and leaves of Bupleuri Radix were dried and crushed into about 20 meshes, and 8 times the weight of 95% ethanol was added to reflux extraction at 80°C for 2 times, each extraction was 4 hours, the extracts were combined, filtered, the ethanol was recovered and Concentrate to a relative density of 1.0-1.1;

[0043] (2) Add 10 times the volume of water to the concentrated solution, leave it for 12 hours, and filter to obtain the filtrate;

[0044] (3) The filtrate obtained in step 2 is passed through a D101 type macroporous resin column, the flow rate is 2ml / min, and water is eluted, and the Molishi reaction is negative to determine the end point of water elution; 70% ethanol is eluted, and the eluate is collected, TLC The end point was determined by detecting that rutin no longer appeared, the ethanol was recovered, and vacuum-dried at 70° C. to obtain 459 g of Bupleurum bupleuri extract powder.

Embodiment 3

[0046] (1) Dry and crush 3000 g of Bupleurum radix stems and leaves into 20 meshes, add 10 times the weight of 75% ethanol and extract twice at 75°C under reflux for 3 hours each time, combine the extracts, filter, recover the ethanol and concentrate , concentrated to a relative density of 1.0-1.1;

[0047] (2) Add 8 times the volume of water to the concentrated solution, leave it for 12 hours, filter to obtain the filtrate;

[0048] (3) The filtrate obtained in step 2 is passed through a D101 type macroporous resin column, the flow rate is 4ml / min, and water is eluted, and the Molishi reaction is negative to determine the end point of water elution; 70% ethanol is eluted, and the eluent is collected, TLC The end point was determined by detecting that rutin no longer appeared, the ethanol was recovered, and vacuum-dried at 70° C. to obtain 735 g of Bupleurum bupleuri extract powder. The preparation of embodiment 4 pharmaceutical composition soft capsules of the present invent...

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Abstract

The invention discloses an extract of bupleurum and preparation method thereof, belonging to the field of traditional Chinese medicine. The preparation method is as follows: drying and grinding the stem and / or leaves of the bupleurum to obtain coarse powder of the bupleurum; adding 75-95% of alcohol solution with 8-10 times of weight for reflux extraction at the temperature of 75-85 DEG C for 1-3times, carrying out extraction for 2-4h every time, combining extracting solution and filtering the extracting solution, recycling and concentrating ethanol until the relative density is 1.0-1.1; adding water with 8-10 times of volume into concentrated solution, placing the mixture for 12h and filtering the mixture, eluting the mixture with D101 macroporous resin column, collecting eluate and vacuum-drying the eluate at the temperature of 70 DEG C to obtain the extract powder of the bupleurum. The invention also discloses a pharmaceutical composition containing the extract of the bupleurum andapplication thereof in preparing drugs for treating liver and nerve diseases.

Description

technical field [0001] The invention belongs to a traditional Chinese medicine extract, in particular to an extract of Bupleurum radix and its preparation method, a pharmaceutical composition containing the extract of Bupleurum radix and its application in the preparation of medicines for treating liver and gallbladder diseases. Background technique [0002] Cholestasis hepatitis belongs to the category of jaundice in traditional Chinese medicine, which can be caused by many reasons, and its main feature is stasis of bile in the liver. The disease has a high incidence rate and poor prognosis, which seriously affects people's working ability and quality of life. [0003] Cholestasis hepatitis is divided into acute and chronic types according to its clinical manifestations. Acute stasis hepatitis can usually heal itself, but if chronic stasis hepatitis is not treated in time, some patients will develop liver cirrhosis, liver failure and liver cancer. [0004] Western medicin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/233A61P1/16A61K135/00A61K127/00
Inventor 蔡光明张卓勇
Owner THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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