Method for degrading crude oil with protozoan infusorian

A protozoa and ciliate technology, applied in the fields of biotechnology and environmental science, can solve the problems of cumbersome screening steps and easy degradation, and achieve the effect of promoting governance and easy cultivation.

Inactive Publication Date: 2009-11-25
HEBEI UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The screening steps are cumbersome, some strains are easy to degenerate, and protozoa are single-celled eukaryotes, which occupy an important position in the water body and soil ecosystem, and are abundant in number. The

Method used

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  • Method for degrading crude oil with protozoan infusorian
  • Method for degrading crude oil with protozoan infusorian
  • Method for degrading crude oil with protozoan infusorian

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Experimental program
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Embodiment 1

[0036] Embodiment 1, application Tetrahymena sp. degradation petroleum:

[0037] A. Separation and purification of ciliate populations from the environment: collect protozoan communities from oil-polluted waters or soil, and use wheat grain culture medium to expand the population in the laboratory. The operation steps are: absorb a tetrahymena Tetrahymena sp. , expand the culture with the wheat grain culture medium to obtain a single population of ciliates;

[0038] Among them: the wheat grain culture solution is to add 1000 ml of water to 5 grams of wheat grains, cook until the grains are cracked, put it for 24 hours, and take its liquid juice.

[0039] B. Domestication of ciliate populations: Add such as 0.5% crude oil in a weight ratio to the above-mentioned isolated and purified ciliate population culture solution, shake and cultivate in a constant temperature shaker, and domesticate for 3-5 days;

[0040] Wherein: the shaking culture condition of constant temperature sha...

Embodiment 2

[0065] Embodiment 2, according to the above-mentioned Tetrahymena method measurement reniform worm (Colpoda sp.) crude oil degradation rate, under the condition that crude oil concentration is respectively 0.25%, 0.5% and 1%, reniform worm (Colpoda sp.) to crude oil The degradation rates were 83.06%, 80.12% and 62.13%, respectively.

[0066] Table 1.

[0067] Degradation Rate of Crude Oil by Tetrahymena sp. and Colpoda sp.

[0068]

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Abstract

The present invention relates to a method for degrading crude oil with protozoan infusorian. The method of the invention is characterized by comprising the following steps: A. separating and purifying infusorian population from the environment; B. domesticating the infusorian population; C. after domesticating, degrading the crude oil by the infusorian population; D. drawing an infusorian population growth curve; and F. after cultivating for 7 days, measuring the crude oil content changing of the control group and the experimental group and calculating the degration rate of the raw oil. The invention firstly finds that the protozoan tetrahymena and colpoda have strong degration capacity for the crude oil. The degration rates can respectively reach up to 68.08% and 83.06% in highest. Furthermore the infusorian has the advantages of: capacity for identifying species through an optical microscope, simple and convenient separation and purifying process, and easy cultivation. As the crude oil degration organism, the infusorian can greatly promote the control of crude oil and the microorganism exploitation of the raw oil and has particular use value for restoring the crude oil pollution through the biological method.

Description

technical field [0001] The invention relates to a method for degrading petroleum by using protozoan ciliates, and belongs to the technical fields of biotechnology and environmental science. Background technique [0002] Petroleum is mainly a mixture of various hydrocarbons (alkanes, cycloalkanes, aromatic hydrocarbons, polycyclic aromatic hydrocarbons) and a small amount of other organic compounds (sulfides, nitrides, alkane acids). With the rapid development of society and economy, Petroleum and its products are widely used in various fields of society. But at the same time, the pollution of oil to the environment has also become the focus of global attention. Petroleum has seriously polluted the environment on which human beings depend, and the pollution has caused serious harm to soil, atmosphere, groundwater, ocean, human beings, animals and plants. Especially the development of offshore oil exploration, development and refining industry, transportation and oil tanker ...

Claims

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Application Information

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IPC IPC(8): C12Q1/64C12R1/90
Inventor 李凤超康现江李彦芹曹卫荣何炜王福强
Owner HEBEI UNIVERSITY
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