Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for degrading crude oil with protozoan infusorian

A protozoa and ciliate technology, applied in the fields of biotechnology and environmental science, can solve the problems of cumbersome screening steps and easy degradation, and achieve the effect of promoting governance and easy cultivation.

Inactive Publication Date: 2009-11-25
HEBEI UNIVERSITY
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The screening steps are cumbersome, some strains are easy to degenerate, and protozoa are single-celled eukaryotes, which occupy an important position in the water body and soil ecosystem, and are abundant in number. They are an important link in the basic food chain and play an important role in the material cycle and energy flow. At present, there is no report on the research on oil degradation by protozoa ciliates at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for degrading crude oil with protozoan infusorian
  • Method for degrading crude oil with protozoan infusorian
  • Method for degrading crude oil with protozoan infusorian

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, application Tetrahymena sp. degradation petroleum:

[0037] A. Separation and purification of ciliate populations from the environment: collect protozoan communities from oil-polluted waters or soil, and use wheat grain culture medium to expand the population in the laboratory. The operation steps are: absorb a tetrahymena Tetrahymena sp. , expand the culture with the wheat grain culture medium to obtain a single population of ciliates;

[0038] Among them: the wheat grain culture solution is to add 1000 ml of water to 5 grams of wheat grains, cook until the grains are cracked, put it for 24 hours, and take its liquid juice.

[0039] B. Domestication of ciliate populations: Add such as 0.5% crude oil in a weight ratio to the above-mentioned isolated and purified ciliate population culture solution, shake and cultivate in a constant temperature shaker, and domesticate for 3-5 days;

[0040] Wherein: the shaking culture condition of constant temperature sha...

Embodiment 2

[0065] Embodiment 2, according to the above-mentioned Tetrahymena method measurement reniform worm (Colpoda sp.) crude oil degradation rate, under the condition that crude oil concentration is respectively 0.25%, 0.5% and 1%, reniform worm (Colpoda sp.) to crude oil The degradation rates were 83.06%, 80.12% and 62.13%, respectively.

[0066] Table 1.

[0067] Degradation Rate of Crude Oil by Tetrahymena sp. and Colpoda sp.

[0068]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a method for degrading crude oil with protozoan infusorian. The method of the invention is characterized by comprising the following steps: A. separating and purifying infusorian population from the environment; B. domesticating the infusorian population; C. after domesticating, degrading the crude oil by the infusorian population; D. drawing an infusorian population growth curve; and F. after cultivating for 7 days, measuring the crude oil content changing of the control group and the experimental group and calculating the degration rate of the raw oil. The invention firstly finds that the protozoan tetrahymena and colpoda have strong degration capacity for the crude oil. The degration rates can respectively reach up to 68.08% and 83.06% in highest. Furthermore the infusorian has the advantages of: capacity for identifying species through an optical microscope, simple and convenient separation and purifying process, and easy cultivation. As the crude oil degration organism, the infusorian can greatly promote the control of crude oil and the microorganism exploitation of the raw oil and has particular use value for restoring the crude oil pollution through the biological method.

Description

technical field [0001] The invention relates to a method for degrading petroleum by using protozoan ciliates, and belongs to the technical fields of biotechnology and environmental science. Background technique [0002] Petroleum is mainly a mixture of various hydrocarbons (alkanes, cycloalkanes, aromatic hydrocarbons, polycyclic aromatic hydrocarbons) and a small amount of other organic compounds (sulfides, nitrides, alkane acids). With the rapid development of society and economy, Petroleum and its products are widely used in various fields of society. But at the same time, the pollution of oil to the environment has also become the focus of global attention. Petroleum has seriously polluted the environment on which human beings depend, and the pollution has caused serious harm to soil, atmosphere, groundwater, ocean, human beings, animals and plants. Especially the development of offshore oil exploration, development and refining industry, transportation and oil tanker ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/64C12R1/90
Inventor 李凤超康现江李彦芹曹卫荣何炜王福强
Owner HEBEI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com