Method for fast analyzing pesticide residue in fruits and vegetables

A technology with many pesticide residues and rapid analysis, applied in the field of food testing, can solve the problems of low sample preparation efficiency, restricting detection efficiency, time-consuming and labor-intensive problems, and achieve the effect of simple operation, less solvent consumption, and avoiding losses

Active Publication Date: 2009-12-09
烟台杰科检测服务有限公司
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Problems solved by technology

[0003] In recent years, the development of testing instruments has been changing with each passing day. However, while the detection efficiency of the instrument has been improved, the traditional sample pretreatment technology has greatly restricted the further improvement of the detection efficiency. For the detection of pesticide residues in fruits and vegetables, the current multi-residue analysis method There are GB/T 19648-2006, GB/T20769-2006 and Japan's Qi analysi

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  • Method for fast analyzing pesticide residue in fruits and vegetables
  • Method for fast analyzing pesticide residue in fruits and vegetables
  • Method for fast analyzing pesticide residue in fruits and vegetables

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[0026] Example 1, a rapid analysis method for multiple pesticide residues in fruits and vegetables. Taking green asparagus as an example, 130 kinds of pesticide mixed standard solutions were prepared, and 0.01 mg / kg and 0.1 mg / kg pesticide standard products were added for recovery. Repeat 4 times for each content level, then extract, weigh 15.0g of uniformly prepared sample, add to a 50mL centrifuge tube containing 1.5g anhydrous sodium acetate and 6g anhydrous magnesium sulfate, then add 15mL 1% acetate acetonitrile solution, vigorously After shaking for 1 min, ultrasonic extraction for 15 min, centrifugation at 5000 r / min for 1 min; purification, transfer 10 mL of acetonitrile extract to 15 mL centrifuge tubes with mixed packing in different proportions, packing 500mg PSA and graphitized carbon with a mass ratio of 1:1. Shake for 1 min, centrifuge at 5000r / min for 1 min, draw 5 mL of the purified solution, concentrate and evaporate to dryness. The volume ratio of acetonitrile an...

Example Embodiment

[0038] Example 2, a rapid analysis method for multiple residues of pesticides in fruits and vegetables. Taking edamame as an example, 28 kinds of pesticide mixed standard solutions were prepared and recovered at a level of 0.02mg / kg. Each content level was repeated 4 times, and then extracted , Weigh 15.0g of the uniformly prepared sample, add it to a 50mL centrifuge tube containing 1.5g of anhydrous sodium acetate and 6g of anhydrous magnesium sulfate, then add 15mL of 1% acetate acetonitrile solution, shake vigorously for 1min, ultrasonic extraction for 15min, 5000r Centrifuge for 1 min per min; purify, transfer 10 mL of acetonitrile extract to 15 mL centrifuge tubes with mixed fillers in different proportions. Fillers are 500 mg graphitized carbon, PSA, and C18 in a mass ratio of 1:2:2. Mix, shake for 1 min, 5000r Centrifuge for 1 min at / min, draw 5 mL of the purified solution, concentrate and evaporate to dryness. The volume ratio of acetonitrile to water is 1:1 to a constant...

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Abstract

The invention discloses a method for fast analyzing pesticide residue in fruits and vegetables, which is implemented by weighing a sample, adding anhydrous sodium acetate and anhydrous magnesium sulfate, adding acetic acid acetonitrile solution, centrifugating after shaking and extracting, putting the centrifugated acetonitrile extract into a centrifugating tube filled with mixed filler, shaking, centrifugating, sucking the cleaning fluid, concentrating and evaporating to be dry, dissolving the mixed solution with the volume ratio of acetonitrile and water being 1:1, and performing UPLC/MS/MS detection. The invention uses fruits and vegetables as matrix, uses the matrix dispersion solid extraction technology to pre-treat the sample to realize fast detection on 130 types of pesticide residue such as avermectins, aldicarb, Cyromazine and the like, having the characteristics of fast and exact detection result, high efficiency and low price.

Description

technical field [0001] The invention belongs to the technical field of food detection methods, and in particular relates to a rapid analysis method for multiple residues of pesticides in fruits and vegetables. Background technique [0002] As countries strengthen the safety inspection of imported food, such as the implementation of Japan's positive list, the limit standards of various agricultural products are getting lower and lower, and the scope of pesticides is getting wider and wider, and the export situation has become very severe. While food safety is getting more and more attention, all countries are trying to find a simple, fast, accurate, efficient, and cheap multi-residue analysis method to meet the needs of food safety in the future. [0003] In recent years, the development of testing instruments has been changing with each passing day. However, while the detection efficiency of the instrument has been improved, the traditional sample pretreatment technology has...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
Inventor 娄喜山付建高洪良
Owner 烟台杰科检测服务有限公司
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