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Method for preparing triterpenoid lucidone C from ganoderma lucidum fermented liquid

A technology of Ganoderma lucidum fermentation and Ganoderma ketone, which is applied in the field of preparing triterpenoid Ganoderma ketone C, can solve the problems of high cost, high difficulty, low yield and the like, and achieves the effect of low cost of raw materials and suitable for large-scale production.

Inactive Publication Date: 2009-12-30
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a method for the existing ganoderma triterpene chizhi ketone C to be prepared from ganoderma lucidum fruiting body with great difficulty, low yield and high cost, as well as the current lack of technical status for preparing ganoderma single triterpene compound A low-cost industrial production method capable of preparing ganoderma triterpene chizhi ketone C from ganoderma lucidum fermentation broth

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0024] First, prepare the ether extract of the traditional Chinese medicine Dung beetle: take the abdominal part of the dung beetle, crush it into a 60-mesh powder, and extract 2.5 oz. h, filtered, and the filtrate evaporated the solvent under reduced pressure to obtain the dung beetle ether extract.

[0025] Then, Ganoderma lucidum preserved by the China General Microorganism Culture Collection and Management Center was used, with the preservation number CCGMC 5.616. Inoculate it in a 1L shake flask with 300mL medium, and ferment for 40 hours at a temperature of 28°C and a rotation speed of 160r / min; 1. Rotary fermentation was finished for 144h under the condition of rotating speed of 140r / min. After centrifugation, the fermentation broth was concentrated to 3 / 5 of the original volume, and 4 times the volume of industrial alcohol was added to remove polysaccharides and proteins. After the supernatant was removed by rotary evaporation, it was extracted with 40 times the volum...

Embodiment 2

[0028] The preparation of the ether extract of the traditional Chinese medicine Dung beetle: Take the abdominal part of the dung beetle, crush it into a 40-mesh powder, extract it with 50 times the volume of ethyl ether acetate (volume ratio: 1:1) at 25°C for 3 hours, and filter , and the filtrate evaporated the solvent under reduced pressure to obtain the ether extract of dung beetle.

[0029]Then, Ganoderma lucidum preserved by the China General Microorganism Culture Collection and Management Center was used, with the preservation number CCGMC 5.616. Inoculate it in a 1L shake flask with 300mL medium, and ferment for 28 hours at a temperature of 30°C and a rotation speed of 130r / min; 140h of rotary fermentation was completed under the condition of rotating speed of 160r / min. After centrifugation, the fermentation broth was concentrated to 3 / 5 of the original volume, and 4 times the volume of industrial alcohol was added to remove polysaccharides and proteins. After the supe...

Embodiment 3

[0032] Preparation of the ether extract of the traditional Chinese medicine Dung beetle: Take the abdominal part of the dung beetle, crush it into a 60-mesh powder, extract it with 50 times the volume of ether-ethyl acetate (volume ratio: 4:1) mixed solution at 25°C for 1.5h , filtered, and the filtrate evaporated the solvent under reduced pressure to obtain the dung beetle ether extract.

[0033] Then, the ganoderma lucidum strain (Ganodema lucidum) preserved by the China General Microorganism Culture Collection and Management Center was used, and the preservation number was CCGMC 5.616. Inoculate it in a 1L shake flask with 300mL medium, and ferment for 70 hours at a temperature of 26°C and a rotation speed of 170r / min; 1. Rotary fermentation was finished for 126h under the condition of rotating speed of 150r / min. After centrifugation, the fermentation broth was concentrated to 3 / 5 of the original volume, and 4 times the volume of industrial alcohol was added to remove poly...

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Abstract

The invention discloses a method for preparing triterpenoid lucidone C from ganoderma lucidum fermented liquid. By replenishing 150 to 400 mg / L of ether extract of dung beetle in the ganoderma lucidum fermented liquid to promote the formation of the triterpenoid lucidone C, and further searching technology of extraction, separation and purification of the triterpenoid lucidone C in the ganoderma lucidum fermented liquid, the method capable of preparing a single lucidone C compound in the ganoderma lucidum fermented liquid with simplicity and low cost is formed. The method has the advantages of simple fermentation process and short fermentation time, and can realize mass production; and the raw material sources are not limited by time, seasons, and the like.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for preparing triterpenoid chizhi ketone C from ganoderma lucidum fermentation liquid. Background technique [0002] Ganoderma lucidum, also known as Ganoderma lucidum, is a famous medicinal fungus in my country. Because it can prevent and treat many diseases, it was once called "immortal grass" in ancient times. In recent years, many scholars in Southeast Asian countries including my country, the United States and Canada have conducted in-depth research on Ganoderma lucidum. [0003] Modern pharmacological research has proved that the key active ingredients of Ganoderma lucidum are polysaccharides and triterpenes of Ganoderma lucidum. Among them, Ganoderma lucidum triterpenoids have anti-tumor, anti-inflammatory, pain-relieving, sedative, detoxifying, liver-protecting, anti-AIDS and other functions, which are the important material basis fo...

Claims

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Application Information

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IPC IPC(8): C12P33/00C12R1/645
Inventor 刘高强王晓玲韩文军
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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