Detection kit for salmonella and shigella in feeds and detection method thereof

Abstract

The invention relates to a detection kit for salmonella and shigella in feeds and a detection method thereof. The kit comprises a detection solution which contains 10mM of Tris.Cl, 50mM of KCl, 25mM of MgCl2, 2.5mM of dNTP, 5U / mu L of Taq DNA polymerase, 10mu M of salmonella primer pair and 10mu M of shigella primer pair. By using the detection kit and the detection method, the salmonella and the shigella in the feeds can be detected with high sensitivity, the detection time consumption is short, the operation is simple and quick, and a large number of labour force and resources can be saved; the detection kit and the detection method are suitable for the requirement of quick detection.

Description

technical field [0001] The invention relates to a detection kit for Salmonella and Shigella in feed and a detection method thereof, in particular to a detection method for detection by PCR-DHPLC technology and used reagents. Background technique [0002] Feed products of animal origin are highly susceptible to microbial contamination, which subsequently contaminates livestock and poultry products, and then causes human diseases through the food chain. If animal-derived feed products come from infected areas with bacteria, dead livestock and poultry, or by-product raw materials that have not been strictly sterilized, they will often cause the spread of animal diseases and cause human diseases through the food chain. Certain pathogenic and relatively pathogenic bacteria can also cause bacterial feed poisoning in livestock and poultry. Microbial contamination of feed is an important way for the spread of zoonotic infectious diseases. The evaluation indicators of microbial pol...

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Problems solved by technology

Although there are many methods, each method has its own advantages and disadvantages: the culture biochemical identification method is greatly affected by human factors and reagents; the automatic biochemical identification of bacteria has good reproducibility, but it is only limited to a few biochemical reactions. , it is particularly difficult to identify similar strains; the detection cost of fluorescent immunoassay (VIDAS) is extremely high, and there is a problem of false results; although the detection cost of PCR-gel electrophoresis detection technology is low, there are difficulties in the post-processing of reaction products. The disadvantage of easy pollution; real-time fluorescent PCR technology has the advantages of strong specificity and high sensitivity, but there are disadvantages such as high detection cost and short storage time of fluorescent probes; while the gene chip method lacks practicability

[0004] In the actual detection, when detecting Salmonella and Shigella, because the enrichment solutions used for the two kinds of bacteria are different, even for the same specimen, the bacteria can only be enriched separately, separated by marking, and identified separately, so that the experiment The workload of the laboratory has doubled. At the same time, due to the different enrichment time of the two, Salmonella is 18-24 hours, and Shigella is 6-8 hours, which brings considerable difficulties to the inspection work arrangement.

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