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Method for preparing lilium total saponin extract by using macroporous resin and application thereof

A technology of lily extract and macroporous resin, which can be used in pharmaceutical combinations, pharmaceutical formulations, plant raw materials, etc., and can solve problems such as low purity

Inactive Publication Date: 2010-01-20
QIANNIANJIAN MEDICINE RES & DEV CO LTD BAOTOU CITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Lily is the dry fleshy scale leaf of Lilium lancifolium Thunb., Lilium brownii var. or Lilium pumilum DC., which has the functions of nourishing yin and moistening the lungs, clearing the heart and calming the nerves. The lily saponin contained in it is the main One of the components, but the purity obtained by traditional methods is far lower than 50%. Except for lily saponins, most of the obtained substances are other substances. Due to the large number of components and the similar physical and chemical properties of many components, It is difficult to separate lily saponins from other components without using special means

Method used

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  • Method for preparing lilium total saponin extract by using macroporous resin and application thereof
  • Method for preparing lilium total saponin extract by using macroporous resin and application thereof
  • Method for preparing lilium total saponin extract by using macroporous resin and application thereof

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preparation example Construction

[0022] 2. Preparation of sample solution

[0023] Weigh the lily medicinal material, extract according to the above-mentioned preferred ethanol extraction process, filter, combine the extracts, and recover the ethanol until it has no alcohol smell. Add 10 times the amount of water to suspend, centrifuge at high speed, and filter under reduced pressure. The filtrate is stored separately for later use. (Concentration is 0.1g / ml based on crude drug amount)

Embodiment 1

[0024] Embodiment 1: 1. Lily is prepared into a crude extract containing the active ingredients of the traditional Chinese medicine

[0025] Accurately weigh 10.0g of lily medicinal material, add 10 times the amount of 80% ethanol to reflux at 70°C for 3 times, each time for 3 hours, combine the filtrates, and dilute to 500ml, dilute the combined filtrates to 500ml with the corresponding solvent, accurately draw 10ml and evaporate to dryness , dissolved in 10ml of water, extracted with 15ml of n-butanol, recovered n-butanol to dryness, dissolved in 10ml of methanol, accurately absorbed 1ml and evaporated to dryness, vanillin-glacial acetic acid method, measured absorbance at 540nm wavelength. Another precision drawing 25ml is placed in the evaporating dish that is dried to constant weight by (Chinese Pharmacopoeia 2005 edition I part, appendix XA) dry method operation of extract, calculate total saponin leaching rate and total saponin purity. The results are shown in Table 1. ...

Embodiment 2

[0046] Accurately weigh 10.0g of lily medicinal material, add 10 times of 65%, 80% and 95% ethanol respectively, and extract under reflux at 70°C for 3 times, each time for 3 hours, and dilute the combined filtrate to 500ml with the corresponding solvent, accurately draw 10ml, evaporate to dryness, and water Dissolve in 10ml, extract with 15ml of n-butanol, recover n-butanol to dryness, dissolve with 10ml of methanol, accurately absorb 1ml and evaporate to dryness, vanillin-glacial acetic acid method, and measure the absorbance at a wavelength of 540nm. Another precise drawing 25ml is placed in the evaporating dish that is dried to constant weight, operates according to the drying method of the extract of "Chinese Pharmacopoeia" (2005 Edition, Part I, Appendix XA), and calculates the total saponin leaching rate and total saponin purity. The results are shown in Table 4.

[0047] Table 4 Extraction of total saponins with different ethanol concentrations (n=6)

[0048]

[0049...

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Abstract

The invention belongs to the field of pharmaceutical research, in particular to a method for preparing lilium total saponin extract brownie by using macroporous resin and the application thereof, aiming at extracting and purifying total lilium saponin from lilium by using macroporous resin and the application of the obtained lilium extract. The obtained lilium extract containing lilium saponins is proved by research to have the function of improving the symptoms of chronic stress depression rats, and can be used for treating human depression. The method for preparing the lilium saponins is convenient, and the prepared lilium saponin has the purity of more than 50 percent; and the invention has low cost and is easily implemented.

Description

Technical field: [0001] The invention belongs to the field of pharmaceutical research, and in particular relates to a method for preparing a lily total saponin extract with a macroporous resin and an application thereof. Background technique: [0002] Lily is the dry fleshy scale leaf of Lilium lancifolium Thunb., Lilium brownii var. or Lilium pumilum DC., which has the functions of nourishing yin and moistening the lungs, clearing the heart and calming the nerves. The lily saponin contained in it is the main One of the components, but the purity obtained by traditional methods is far lower than 50%. Except for lily saponins, most of the obtained substances are other substances. Due to the large number of components and the similar physical and chemical properties of many components, It is difficult to separate lily saponins from other components without using special means. Invention content: [0003] The purpose of the present invention is to make up for the deficiencie...

Claims

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Application Information

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IPC IPC(8): A61K36/8967A61P25/24A61K127/00
Inventor 李卫民朱贺年贾金良
Owner QIANNIANJIAN MEDICINE RES & DEV CO LTD BAOTOU CITY
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