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Immune fusion protein and gene encoding same and application thereof

A gene and protein technology, applied in the field of immune fusion protein and its coding gene and application, can solve the problem of weak complement fixation ability, achieve high affinity, huge economic and social benefits, and broad clinical application prospects

Active Publication Date: 2010-01-27
BEIJING JINGYI TAIXIANG TECH DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

IgG1 and IgG3 can effectively bind complement C1q and induce the classic activation pathway of complement, while IgG2 has a weak ability to fix complement

Method used

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  • Immune fusion protein and gene encoding same and application thereof
  • Immune fusion protein and gene encoding same and application thereof
  • Immune fusion protein and gene encoding same and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1. Acquisition of the immune fusion protein FcεRIα / IgG2 and its functional analysis

[0043] 1. Acquisition of the immune fusion protein FcεRIα / IgG2

[0044] 1. Optimization and synthesis of expression gene of immune fusion protein FcεRIα / IgG2

[0045] Artificially synthesize the DNA fragment shown in Sequence 1, use the DNA fragment as a template, and perform PCR amplification with the following primers:

[0046] Upstream primer: 5-CCG CTCGAG CCGCCACCATGGAGAC-3;

[0047] Downstream primer 5-CCG GAATTC TTACTTTTCCAGGAGACAGGGACAGG-3,

[0048] The underlined parts are the restriction sites of XhoI and EcoRI respectively, which were synthesized by Beijing Aoke Biotechnology Co., Ltd.

[0049] The reaction conditions of PCR amplification were: pre-denaturation at 95°C for 5 minutes; denaturation at 94°C for 45 s, annealing at 56°C for 45 s, extension at 72°C for 2 min, and 30 cycles of amplification; and extension at 72°C for 10 minutes. PCR amplified product...

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Abstract

The invention discloses a protein. The provided protein is the following protein (1) or (2): the protein (1) is formed by an amino acid sequence shown by a sequence 2 in a sequence table; and the protein (2) is formed by the amino acid sequence of the sequence 2 in the sequence table through the substitution and / or deletion and / or addition of one or several amino acid residues, can be combined with IgE molecules and is derived from the protein (1). The protein has higher affinity with the IgE; Fc epsilonR I alpha and IgG2 are both derived from humanized protein, and the protein has no antigenicity, does not need humanized reconstruction, has a longer half life, and has the molecular weight of 170kDa which is larger than the molecular weight of sFcepsilonR I alpha. The protein has potential value on treating anaphylactic diseases including asthma, allergic rhinitis, atopic dermatitis, food allergy and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an immune fusion protein and its coding gene and application. Background technique [0002] The incidence of allergic diseases such as asthma, allergic rhinitis, atopic dermatitis and food allergy has been increasing year by year in the past 20 years. As far as asthma alone is concerned, there are about 100-150 million people in the world suffering from asthma, which seriously affects the physical and mental health of patients and can cause about 180,000 deaths every year. The global cost of treating asthma exceeds that of AIDS and tuberculosis combined. IgE molecules are key molecules involved in allergic diseases. The allergen binds to the IgE that has been bound to the surface of the effector cell membrane, causing the cross-linking of the IgE receptor on the cell surface, hydrolyzing the phosphoinositide, and the cytoplasmic Ca 2+ The increase in concentration will lead to deg...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62C12N15/63C12N1/00C12N5/10C12P21/02A61K38/17A61K47/48A61K48/00A61P11/06A61P11/02A61P17/00A61P37/08A61K47/68
Inventor 胡品良马金伟程红褚学者李先钟扈艳红赵天贵喻志爱林峰何丽华白先宏
Owner BEIJING JINGYI TAIXIANG TECH DEV
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