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Amino plastic substrate, and preparation method and application thereof

A technology for plastic sheets and substrates, which is used in biochemical equipment and methods, determination/inspection of microorganisms, etc.

Active Publication Date: 2010-01-27
CAPITALBIO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with plastic materials, glass materials are not easy to process and shape, which makes them particularly powerless in the field of biochips such as microfluidics based on various microstructures

Method used

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  • Amino plastic substrate, and preparation method and application thereof
  • Amino plastic substrate, and preparation method and application thereof
  • Amino plastic substrate, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1, prepare two-dimensional amino plastic substrate with aminosilanization method in aqueous solution

[0057] 1. Pretreatment of the film base: Wash the plastic film base with PS deionized water, dry it, and carry out O 3 Treat for 3h or Plasma treatment for 2min under aerobic conditions.

[0058] 2. Aminosilanization: place the plastic sheet base pretreated in step 1 in an aqueous solution of APTS, the concentration of APTS in the solution is 0.01% (volume percentage), and react at 70°C and 120rpm shaking conditions for 30 hours; wash with deionized water and dry to obtain a two-dimensional amino plastic substrate.

[0059] The surface of the plastic sheet is cleaned and modified, and the surface of the plastic sheet is distributed with amino groups by reacting with APTS to obtain an aminoplast substrate (two-dimensional aminoplast substrate).

[0060] The plastic substrate PS was replaced by PC or PMMA, and the above experiment was carried out, and a two-...

Embodiment 2

[0061] Embodiment 2, prepare two-dimensional amino plastic substrate with aminosilanization method in aqueous solution

[0062] 1. Pretreatment of film base:

[0063] With experiment 1 in embodiment 1.

[0064] 2. Aminosilanization: Place the plastic sheet base pretreated in step 1 in an aqueous solution of APTS, the concentration of APTS in the solution is 10% (volume percentage), and react 0.5% at 15°C and 120rpm shaking conditions. hours; wash with deionized water and dry to obtain a two-dimensional amino plastic substrate.

[0065] The plastic substrate PS was replaced by PC or PMMA, and the above experiment was carried out, and a two-dimensional aminoplastic substrate was also obtained.

Embodiment 3

[0066] Embodiment 3, two-dimensional amino plastic substrate is prepared by aminosilanization method in aqueous solution

[0067] 1. Pretreatment of film base:

[0068] With experiment 1 in embodiment 1.

[0069] 2. Amino silanization: place the plastic sheet base pretreated in step 1 in an aqueous solution of APTS, the concentration of APTS in the solution is 0.5% (volume percentage), and react at 35°C and 120rpm shaking conditions for 5 hours; wash with deionized water and dry to obtain a two-dimensional amino plastic substrate.

[0070] The plastic substrate PS was replaced by PC or PMMA, and the above experiment was carried out, and a two-dimensional aminoplastic substrate was also obtained.

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Abstract

The invention discloses an amino plastic substrate, and a preparation method and application thereof. The preparation method of a biochip substrate comprises the following steps: connecting an amino group with the surface of the plastic substrate and obtaining the substrate. The surface of the substrate is connected with the amino group. Proved by tests, a biochip with excellent performance is prepared on plastic material by the preparation method. The plastic material has strong plasticity and convenient forming, and processing and various micro-structures and functional units can be designed; therefore, the preparation method can be applied to the field of the biochip, such as micro-fluidic control, and the like.

Description

technical field [0001] The invention relates to an amino plastic substrate and its preparation method and application. Background technique [0002] At present, chip technology has become a vital technology for large-scale and high-throughput research in biology. With the development of biochip technology, the material technology and surface chemical technology related to its preparation also need to be continuously improved and improved, which is mainly reflected in the preparation of biochip substrates of different materials. At present, the rigid inorganic material-glass is mainly used for the preparation of biochip substrates, but the substrates made of glass are mainly used for the preparation of passive biochips in which all reactions are carried out by random diffusion. Active chips for the purpose of manipulating molecules and cells and controlling reactions have rarely been adopted. Compared with plastic materials, glass materials are not easy to process and shape...

Claims

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Application Information

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IPC IPC(8): C08J7/12C12Q1/68
Inventor 甘五鹏王佳吕品王素珍周玉祥程京
Owner CAPITALBIO CORP
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