Two-dimensional scanning detection method and device of surface plasma resonance biochemical analysis
A surface plasmon and two-dimensional scanning technology, which is applied in the field of plasma resonance sensing, biochemical analysis, scanning and detection, can solve problems such as incompatibility
Inactive Publication Date: 2011-01-05
JINAN UNIVERSITY
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At present, the research on high-channel SPR biochemical analyzers is mainly focused on the imaging SPR structure (Cui Dafu et al., "Research on high-throughput, multi-component surface plasmon resonance biochemical analysis system", Progress in Natural Science, 2007, 17(1) :93-98), but the imaging SPR biochemical analyzer is far inferior to the single-beam single-point detection SPR biochemical analyzer in terms of detection accuracy
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The invention relates to a surface plasma resonance biochemical sensing scanning detection technology. The invention aims at a surface plasma resonance biochemical sensor based on resonance angle detection and provides a two-dimensional scanning detection method and a two-dimensional scanning detection device of surface plasma resonance biochemical analysis. The called two-dimensional scanning detection is single beam two-dimensional scanning detection for a two-dimensional sensing chip. In virtue of elaborate design of a rotation axis position and a two-dimensional scanning path for angle scanning, beams are incident on an incidence point on the detected chip without deflecting from a detected sample point in the angle scanning process and the two-dimensional scanning process, thus overcoming a difficult problem that the beam incidence point easily deflects from the detected sample for the surface plasma resonance biochemical sensor based on resonance angle detection in the two-dimensional scanning detection process, which is of great significance to improve sample density of the sensing chip for the two-dimensional scanning detection and realize high-precision and high-throughput detection, and has wide application prospect in biochemical analysis.
Description
Two-dimensional scanning detection method and device for surface plasmon resonance biochemical analysis Technical field The invention relates to a surface plasmon resonance sensor biochemical analysis scanning detection technology. Background technique Since the Surface Plasmon Resonance (SPR) technology was used in the sensor research field in the 1980s, it has gradually become a research hotspot in the international sensor field. The SPR sensor has the characteristics of label-free, real-time dynamic detection, fast, sensitive, and non-damaging, and has unique advantages in determining the reaction kinetic constants and characteristics during the interaction of biological macromolecules. Judging from the current research status, SPR sensing technology has the potential to be widely used in many fields such as biology, medicine, chemistry, drug screening, environmental monitoring, and food safety. There are two surface plasmon sensing methods: one is to detect the resonance ...
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IPC IPC(8): G01N21/55G01N21/552
Inventor 钟金钢张冼华
Owner JINAN UNIVERSITY