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Enzyme immobilized electrode, fuel cell, electronic equipment, enzyme reaction utilization apparatus, and enzyme immobilized base

A technology of enzyme immobilization and fuel cells, applied in biochemical fuel cells, fuel cells, immobilized on or in inorganic carriers, etc., can solve problems such as inability to obtain energy conversion efficiency and consume chemical energy

Inactive Publication Date: 2010-02-03
SONY CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, in microorganisms and batteries, there are many unnecessary functions beyond the intended reactions (i.e., conversion from chemical energy to electrical energy)
Therefore, in the above-mentioned method, chemical energy is consumed in undesired reactions, and sufficient energy conversion efficiency cannot be obtained

Method used

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  • Enzyme immobilized electrode, fuel cell, electronic equipment, enzyme reaction utilization apparatus, and enzyme immobilized base
  • Enzyme immobilized electrode, fuel cell, electronic equipment, enzyme reaction utilization apparatus, and enzyme immobilized base
  • Enzyme immobilized electrode, fuel cell, electronic equipment, enzyme reaction utilization apparatus, and enzyme immobilized base

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Proteins are generally classified into three types: membrane proteins distributed in cell membranes, water-soluble proteins distributed in extracellular or intracellular solutions, and membrane-bound proteins in contact with cell membranes. Among them, membrane proteins and membrane-bound proteins are easily introduced into liposomes. Meanwhile, some proteins used for biofuels are usually distributed in intracellular or extracellular solution and have a relatively small amount of fat-soluble sites.

[0071] Meanwhile, as a configuration of phospholipid aggregates, spherical liposomes in the form of phospholipid bilayers are well known. Most membranes constituting living things are composed of liposomes, which are phospholipid aggregates, and they are very good reaction sites for keeping enzymes, proteins (especially membrane proteins) stable and highly active. Therefore, in Example 1, first, fat-soluble sites were introduced into the two enzymes 13 and 14, which were t...

Embodiment 2

[0081] As the electrode 11, a porous carbon electrode was used. The porous carbon electrode was activated with an ultraviolet (UV) ozone treatment unit to make the surface hydrophilic, and then immersed in a phosphate buffer containing about 500 μg / mL to 5 mg / mL enzyme and kept overnight. Then, rinse with phosphate buffered saline and dry. Thus, a porous carbon electrode having an enzyme as the intermediate layer 18 was obtained.

[0082] Next, liposomes including ANQ as electron mediator 16, diaphorase and glucose dehydrogenase as enzymes 13 and 14, and NADH as coenzyme were added dropwise to the porous carbon with enzyme as intermediate layer 18. electrodes and then dry. Thus, an enzyme-immobilized electrode was produced. As a control, an enzyme-immobilized electrode was manufactured by activating a porous carbon electrode using a UV ozone treatment device to make the surface hydrophilic, and then dropwise adding a liposome solution containing an enzyme or the like (Compa...

Embodiment 3

[0088] As the electrode 11, a porous carbon electrode was used. The porous carbon electrode was activated with an ultraviolet (UV) ozone treatment unit to make the surface hydrophilic, and then immersed in a phosphate buffer containing about 500 μg / mL to 5 mg / mL enzyme and kept overnight. Then, rinse with phosphate buffered saline and dry. Thus, a porous carbon electrode having an enzyme as the intermediate layer 18 was obtained.

[0089]Next, ANQ as electron mediator 16, diaphorase and glucose dehydrogenase as enzymes 13 and 14, NADH as coenzyme, PLL as polycation, PLL as polyanion, and The solution of PAAcNa was added dropwise onto the porous carbon electrode with the enzyme as an intermediate layer 18 and then dried. Thus, an enzyme-immobilized electrode was produced. As a control, the enzyme-immobilized electrode of Comparative Example 1 was used to compare electrochemically. In the test, a potentiostatic test and a cyclic voltammetry test at 0.1 V were performed using...

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Abstract

An enzyme-immobilized electrode is provided and includes an electrode composed of porous carbon or the like, a phospholipid layer on the electrode (11), and enzymes immobilized onto the phospholipid layer. The enzymes are, for example, diaphorase and glucose dehydrogenase. An intermediate layer composed of a protein or the like may be provided between the electrode and the phospholipid layer. By using the enzyme-immobilized electrode as a negative electrode or a positive electrode in a fuel cell using an enzyme, one or a plurality of types of enzymes can be immobilized at optimal positions onthe electrode, and thus, there are provided a highly efficient enzyme-immobilized electrode and a highly efficient fuel cell using the enzyme-immobilized electrode.

Description

technical field [0001] The present invention relates to enzyme-immobilized electrodes, fuel cells, electronic equipment, devices using enzyme reactions, and enzyme-immobilized substrates, and the present invention is suitable for use in, for example, biofuel cells using enzymes, and using such biofuel cells as power sources Various equipment, devices, systems, etc. Background technique [0002] A fuel cell has a structure in which a positive electrode (oxidant electrode) and a negative electrode (fuel electrode) face each other with an electrolyte (proton conductor) in between. In a conventional fuel cell, the fuel (hydrogen) supplied to the negative electrode is oxidized and split into electrons and protons (H + ); these electrons are delivered to the negative electrode; and H + Move through the electrolyte to the positive electrode. At the positive electrode, H + Reacts with oxygen supplied from the outside and electrons transferred from the negative electrode through ...

Claims

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Application Information

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IPC IPC(8): H01M8/16C12N11/02G01N27/327H01M4/90H01M8/00
CPCH01M4/90H01M8/16Y02E60/527C12N11/02C12N11/14Y02E60/50
Inventor 角田正也酒井秀树中川贵晶户木田裕一
Owner SONY CORP
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