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Preparation method of whey protein powder containing low beta-lactoglobulin

The technology of whey protein powder and lactoglobulin is applied in the directions of food preparation, protein food composition, milk protein composition, etc., which can solve the problems of expensive equipment, low output, low β-Lg removal rate, etc. The effect of low cost and low cost

Inactive Publication Date: 2010-02-10
黑龙江省乳品工业技术开发中心
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention aims to solve the problems of expensive equipment, low output and low removal rate of β-Lg in the existing method for preparing whey protein, and provides a preparation method of whey protein powder containing low β-lactoglobulin method

Method used

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  • Preparation method of whey protein powder containing low beta-lactoglobulin
  • Preparation method of whey protein powder containing low beta-lactoglobulin

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specific Embodiment approach 1

[0008] Specific embodiment one: the method for preparing the whey protein powder containing low β-lactoglobulin in this embodiment is as follows: one, the whey protein concentrate is dissolved in the sodium hexametaphosphate solution whose concentration is 0.15mol / L, and then use The citric acid with a concentration of 0.05mol / L adjusts the pH to 3.9-4.0, and then stirs at a temperature of 0-80°C for 30-60 minutes to obtain a mixed solution; Centrifuge at 5000-6500r / min for 30min, discard the supernatant to obtain precipitate A; 3. Wash the precipitate A twice with NaCl solution with a volume fraction of 5%-10%, and after each washing, use temperature Centrifuge at 4°C and a centrifugal rate of 9000r / min for 20 minutes, discard the supernatant to obtain precipitate B; 4. Dissolve precipitate B in pH 7.5 and concentration 0.10 at a temperature of 30-50°C ~0.40mol / L of CaCl 2 solution, and then centrifuged at a temperature of 4°C and a centrifugal rate of 9000r / min for 20min to...

specific Embodiment approach 2

[0009] Embodiment 2: This embodiment is different from Embodiment 1 in that the temperature in step 1 is 20-60°C. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0010] Embodiment 3: This embodiment is different from Embodiment 1 in that the temperature in step 1 is 40°C. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a preparation method of whey protein powder containing low beta-lactoglobulin and relates to the preparation method of the whey protein powder. The preparation method can solvethe problems of expensive equipment, low output and low removal rate of beta-Lg of the existing preparation method of whey protein. The method comprises the steps of 1, preparing mixed liquid; 2, preparing a sediment A; 3, preparing a sediment B; 4, preparing supernatant liquid; 5, preparing solution in a dialysis tubing; and 6, adopting the freeze-drying method or the spray-drying method on thesolution in the dialysis tubing, thereby obtaining the whey protein powder containing low beta-lactoglobulin. The obtained whey protein powder containing low beta-lactoglobulin improves the output by10%-15% and reduces the removal rate of beta-Lg by 12 times. The prepration method has low cost, and the used equipment is simple and low in price.

Description

technical field [0001] The invention relates to a preparation method of whey protein powder. Background technique [0002] For babies, breast milk is undoubtedly the best food, because breast milk contains nutrients necessary for baby growth to ensure baby growth and development, improve immunity and prevent infectious diseases. However, due to working conditions, occupations, diseases and other reasons, the current situation of breastfeeding is not optimistic, and the phenomenon of breastmilk deficiency is very common. Cow's milk is often used as a substitute for human milk because of its wide source and close composition to breast milk. However, there are still certain differences between cow's milk and human milk. For example, feeding newborn babies with cow's milk often causes many incompatibilities. Studies have shown that: β-lactoglobulin (β-Lg) is the main allergen in milk, which can cause allergic reactions in infants and cause many discomforts in infants themselve...

Claims

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Application Information

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IPC IPC(8): A23J1/20A23L1/305A23L1/015
Inventor 刘宁张楠楠刘鹏张微
Owner 黑龙江省乳品工业技术开发中心
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