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Artificial culture method of xylaria gracillima

A technology of artificial cultivation and carbon horn bacteria, which is applied in the direction of botany equipment and methods, fungal products, fertilizer mixtures, etc., to achieve the effects of improving stability, low cost, and large growth

Active Publication Date: 2011-01-19
浙江三禾生物工程股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the problem that it is difficult to cultivate the slender carbon horn bacteria and improve the stability of the slender carbon horn bacteria. artificial culture of bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Step 1: Sterilize the 500ml culture bottle at 120°C for 10 hours, and let the culture bottle cool down naturally;

[0030] Step 2: Fill in the mixed culture medium occupying 1 / 3 of the volume of the culture bottle, and then sterilize it together at 120°C for 10 hours, and then let the culture bottle cool down naturally;

[0031] The formula of the mixed culture medium is: 100 grams / liter of corn flour, 100 grams / liter of soybean meal, 20 grams / liter of fish bone meal, 20 grams / liter of glucose, 5 grams / liter of silkworm chrysalis powder, 1 gram of potassium dihydrogen phosphate / liter, dipotassium hydrogen phosphate 0.5 g / liter, calcium carbonate powder 10 g / liter;

[0032] Step 3: Inject the C. tenurans strains in a sterile environment, seal the culture bottle after inoculation, and increase the volume ratio of carbon dioxide gas in the container from 0.03% to 1%;

[0033] Step 4: Put the bottle for closed culture in a culture environment at 20°C, and let the C. ...

Embodiment 2

[0037] Step 1: Sterilize the 700ml culture bottle at 120°C for 10 hours, and let the culture bottle cool down naturally;

[0038] Step 2: Fill in the mixed culture medium occupying 1 / 3 of the volume of the culture bottle, and then sterilize it together at 120°C for 10 hours, and then let the culture bottle cool down naturally;

[0039] The formula of the mixed culture medium is: 100 grams / liter of corn flour, 100 grams / liter of soybean meal, 20 grams / liter of fish bone meal, 20 grams / liter of glucose, 10 grams / liter of silkworm chrysalis powder, 2 grams of potassium dihydrogen phosphate / liter, dipotassium hydrogen phosphate 1 g / liter, calcium carbonate powder 20 g / liter;

[0040] Step 3: Insert the C. tenurans strains in a sterile environment, seal the culture bottle after inoculation, and increase the volume ratio of carbon dioxide gas in the container from 0.03% to 2%;

[0041] Step 4: Put the bottle for closed culture in a culture environment of 20°C, and let the C. ...

Embodiment 3

[0045] Step 1: Sterilize the 600ml culture bottle at 120°C for 10 hours, and let the culture bottle cool down naturally;

[0046] Step 2: Fill in the mixed culture medium occupying 1 / 3 of the volume of the culture bottle, and then sterilize it together at 120°C for 10 hours, and then let the culture bottle cool down naturally;

[0047] The formula of described mixed culture medium is: 100 grams / liter of corn flour, 100 grams / liter of soybean meal, 20 grams / liter of fish bone meal, 20 grams / liter of glucose, 8 grams / liter of silkworm chrysalis powder, 1.5 grams / liter of potassium dihydrogen phosphate liter, dipotassium hydrogen phosphate 0.8 g / l, calcium carbonate powder 15 g / l;

[0048] Step 3: Inject the C. tenurans strains in a sterile environment, seal the culture bottle after inoculation, and increase the volume ratio of carbon dioxide gas in the container from 0.03% to 1.5%;

[0049] Step 4: Put the bottle for closed culture in a culture environment at 20°C, and let the ...

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PUM

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Abstract

The invention relates to an artificial culture method of bacteria, which discloses an artificial culture method of xylaria gracillima. A culture bottle is sterilized at the temperature of 120 DEG C and then naturally cooled down, a mixed culture medium is arranged in the culture bottle, the culture bottle is sterilized at the temperature of 120 DEG C again and then naturally cooled down, and the culture bottle is closed after xylaria gracillima strains are inoculated; after the xylaria gracillima is cultured at the temperature of 20 DEG C, a natural culture method is used for obtaining maturexylaria gracillima sporocarp which is washed by sterile water and sterilized by alcohol with the concentration of 70% and then the sporocarp is chopped into block-shaped clinohedrals in the length of3mm, the clinohedrals are inoculated on the improved culture medium, after hoar hyphae grow from the inoculation culture medium, culturing is continued for 1 to 2 days, and thus xylaria gracillima mycelia are obtained, the mycelia are slowly formed by growing development to become xylaria gracillima finally. The technique solves the problem that xylaria gracillima strains are difficult to be cultured into strains for production, and has the advantages of simple process, great mass growth, high efficiency, low cost and good social benefits and application prospect.

Description

technical field [0001] The invention relates to a method for artificially cultivating bacteria, in particular to a method for artificially cultivating Pyrethrocystus. Background technique [0002] Xylariaceae (Xylariaceae) is a family of Fungi, Ascomycota, Sclerotinia, and Spheroides. As a rare species, Xylariaceae is widely distributed in China, but its output is small. Natural fruiting bodies are as expensive as gold. For example, the artificially cultivated C. nigriscens already on the market are strains isolated from natural C. nigriscens. At present, the existing C. arbuscules include C. arbusci and C. racemosa. , Xylaria gracillima, Xylaria gracillima, etc., but the types of Xylaria gracillima are still insufficient at present, and further research and development are needed. Xylaria gracillima was preserved in 2006 The General Microbiology Center of China Microbiological Culture Collection Management Committee, the registration number is: CGMCC No.1625, it is a symb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04A01H15/00C05G1/00C05G3/00
Inventor 朱志熊
Owner 浙江三禾生物工程股份有限公司
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