Tumor dual target liposome mediated by integrin and preparation method thereof

A technology of liposome and liposome composition, which is applied in the directions of liposome delivery, anti-tumor drugs, medical preparations of inactive ingredients, etc. , the effect of inhibiting tumor growth

Inactive Publication Date: 2010-02-24
PEKING UNIV
View PDF0 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many experiments have proved that ordinary hidden liposomes cannot enter tumor cells through phagocytosis, so although lon

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tumor dual target liposome mediated by integrin and preparation method thereof
  • Tumor dual target liposome mediated by integrin and preparation method thereof
  • Tumor dual target liposome mediated by integrin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the preparation of the long circulation liposome of the PHSCNK modification of encapsulating doxorubicin

[0044] Take hydrogenated soybean lecithin, cholesterol, dipalmitoylphosphatidylethanolamine, OHC-PEG2000-CHO (molar ratio is 15:10:5:10), put in a round bottom flask, add appropriate amount of chloroform:methanol (10:1), After ultrasonic dissolution, heat in a water bath and vacuum rotary evaporation to remove the organic solvent to form a uniform transparent film. Draw an appropriate amount of ammonium sulfate solution (123mM, pH 5.4) into a round-bottomed flask, vortex and oscillate to completely shed the lipid film, and sonicate in a water bath until light blue opalescence appears. Pass the prepared blank liposomes through a Sephadex G50 column, elute with phosphate buffered saline (PBS, pH 7.4), collect the liposome fraction, and preheat the collected blank liposomes in a water bath at 75°C. Add doxorubicin stock solution and incubate for 10 minu...

Embodiment 2

[0048] Example 2. In vitro cell-targeting evaluation of PHSCNK-modified long-circulating liposomes loaded with doxorubicin

[0049] The uptake of doxorubicin by cells and the distribution of doxorubicin in cells were investigated by laser confocal experiment. Human umbilical vein endothelial cells or melanoma cells were placed in CO with PL-DOX and PHSCNK-PL-DOX, respectively. 2 Incubate in the incubator for 2 hours, rinse with cold PBS buffer for 3 times in sequence, fix the tissue cells with fixative solution, then stain the nucleus with Hoechst 33258 for 10 minutes, rinse with PBS for 3 times, seal with PBS, refrigerate in the dark, and use laser confocal Microscopic determination. The experimental results showed that PHSCNK-modified doxorubicin long-circulating liposomes could significantly increase the drug uptake by endothelial cells and tumor cells.

Embodiment 3

[0050] Example 3, Pharmacokinetics of PHSCNK-modified long-circulating liposomes loaded with doxorubicin and distribution in tumor tissues

[0051] Since the present invention combines the long-circulation liposome technology and the method of promoting intracellular transport to improve the curative effect of anti-cancer drugs, it is required to possess the characteristics of long-circulation liposomes, that is, to delay the clearance of drugs in plasma and the delivery of drugs to tumor tissues. and transport in tumor cells. In this example, C57BL / 6 mice inoculated with melanoma B1610 were used as an animal model, and the experimental results after tail vein injection of doxorubicin preparation.

[0052] Reference preparations: doxorubicin long-circulation liposome, free doxorubicin solution.

[0053] Test preparation: PHSCNK-modified long-circulating liposomes loaded with doxorubicin.

[0054] Experimental animals: C57BL / 6 mice inoculated with mouse-derived melanoma B16F1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a tumor dual target liposome mediated by integrin and a preparation method thereof. The composition of the invention comprises the components of an antitumor medicament, polypeptide with tumor new vessel target function and tumor cell target function, polyethylene glycol and derivates thereof and a vector for preparing liposome medicaments.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical dosage forms and preparations, and in particular relates to a targeted drug delivery system loaded with anticancer drugs for injection. Simultaneously with polyethylene glycol (PEG) and the integrin ligand containing phenylalanine-histidine-cysteine-serine-asparagine (PHSCN) sequence the surface of liposome is modified, after modification The liposomes can deliver anti-cancer drugs to tumor neovascular endothelial cells and tumor cells at the same time, thereby enhancing the therapeutic effect of tumors. Background technique: [0002] Malignant tumors have always been an important disease that plagues human beings, and there is currently no cure for cancer. The traditional therapy for malignant solid tumors is surgical resection of the tumor followed by chemotherapy with antineoplastic drugs. Most chemotherapeutic drugs are non-selective, and these drugs also have a killing effect on norm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K9/127A61K45/00A61K47/42A61P35/00A61K47/10
Inventor 张强代文兵王坚成张烜
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap