Method for extracting sheep embryo bioelement from sheep placenta by utilizing low temperature of liquid nitrogen
A technology for sheep placenta and sheep placenta, which is applied in the field of extracting sheep placenta from sheep placenta by utilizing liquid nitrogen low-temperature oxygen discharge and extracting sheep placenta, can solve the loss of active components, the reduction of sheep placenta extraction rate, and the activity of sheep placenta. Loss of ingredients and other issues to achieve the effect of preventing pollution
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Embodiment 1
[0008] Embodiment 1: Select 1.5Kg of fresh and healthy sheep placenta as raw material, rinse it with deionized water for use; add liquid nitrogen while pulverizing the sheep placenta, and grind the ambient temperature at 25°C; The mixing ratio of slurry and water is 1:3, the temperature is 40°C, the time is 60 minutes, the pH is naturally hydrolyzed; then extracted with 0.9% normal saline; filtered with inorganic membrane; sterilized at 450 atmospheres, and the sheep placenta polypeptide in the extract is detected for immunity The content of globulin active ingredient is 83.9%.
Embodiment 2
[0009] Embodiment 2: Use 1.8Kg of fresh and healthy sheep placenta as raw material, rinse it with deionized water for use; add liquid nitrogen while pulverizing the sheep placenta, and grind the ambient temperature at 25°C; The mixing ratio of slurry and water is 1:4, the temperature is 40°C, the time is 50 minutes, the pH is naturally enzymatically hydrolyzed; then extracted with 0.9% normal saline; filtered with inorganic membrane; The protein active ingredient content is 82.5%.
Embodiment 3
[0010] Embodiment 3: Use 1.4Kg of fresh and healthy sheep placenta as raw material, wash it with deionized water for use; add liquid nitrogen while pulverizing the sheep placenta, and grind the ambient temperature at 25°C; The mixing ratio of slurry and water is 1:3 water, temperature 45°C, time 60 minutes, pH natural enzymatic hydrolysis; then extract with 0.9% normal saline; inorganic membrane filtration; sterilize at 450 atmospheres, detect sheep placenta polypeptide in the extract The content of immunoglobulin active ingredient is 84.6%.
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