Method for extracting and separating D-fraction polysaccharide from fruit bodies or mycelia of polyporus frondosus

A separation method and mycelium technology, which are applied in the field of extraction and separation of Grifola frondosa fruiting bodies or mycelium polysaccharide components, can solve problems such as complicated purification steps, and achieve the effects of high purity and convenient operation.

Active Publication Date: 2010-03-03
ZHEJIANG FANGGE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, those methods described above are not suitable for the preparation of a large amount of medicines and the efficient preparation of health foods from limited resources, because the purification steps of these methods are rather complicated, and the obtained products contain substances that suppress the immunopotentiating activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The extraction of embodiment 1 Grifola frondosa crude polysaccharide

[0021] Weigh 15Kg of Grifola frondosa fruit body or mycelium fine powder, add 500L of water, extract at 100°C for 1h, centrifuge at 8000rpm for 20min, concentrate the supernatant to a density of 1.04, add 3 times the amount of 95% ethanol, and stand at 4°C Overnight, filtered, and the resulting precipitate was preserved in a small amount of ethanol for future use.

Embodiment 290

[0022] The separation and purification of the Grifola frondosa polysaccharide D component of the molecular weight of 2.9 million Daltons

[0023] Grifola frondosa polysaccharide extract was redissolved in 30 times hot water, centrifuged, added 30% PTOH until no precipitation occurred, adjusted to PH = 11, kept at 4°C for 48 hours, centrifuged, discarded supernatant to obtain precipitate; used for precipitation Wash 2 times with 20% acetic acid of 2 times the volume, wash 2 times with 90% ethanol, redissolve with 0.5 NaOH solution, centrifuge, deproteinize the supernatant 3 times with sevag method; add ethanol to the supernatant to ethanol concentration Redissolve the precipitate with 0.2M NaOH solution, add ethanol to make the ethanol concentration 35%, precipitate, centrifuge, take the precipitate and redissolve it with 0.2M NaOH solution, add ethanol to make the ethanol concentration 30%, precipitate , centrifuged, and the precipitate was washed with 90% and absolute ethanol...

Embodiment 3115

[0024] The separation and purification of the Grifola frondosa polysaccharide D component of embodiment 31.15 million Dalton molecular weight

[0025] Grifola frondosa polysaccharide extract was redissolved by adding 30 times of hot water, centrifuged, added 30% CTAOH until no precipitation occurred, adjusted to PH=12, kept at 4°C for 48 hours, centrifuged, discarded supernatant to obtain precipitate; used for precipitation Wash 2 times with 20% acetic acid of 2 times the volume, wash 2 times with 90% ethanol, redissolve with 0.5 NaOH solution, centrifuge, deproteinize the supernatant 3 times with sevag method; add ethanol to the supernatant to ethanol concentration 40% to make the precipitate, the precipitate is redissolved with 0.2M NaOH solution, add ethanol to make the ethanol concentration 30%, precipitate, centrifuge, take the precipitate and redissolve with 0.2M NaOH solution, add ethanol to make the ethanol concentration 25%, precipitate , centrifuged, the precipitate ...

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Abstract

The invention relates to a method for extracting and separating D-fraction polysaccharide from fruit bodies or mycelia of polyporus frondosus, which is characterized in that: the fruit bodies or mycelia of the polyporus frondosus is crushed, extracted by hot water, precipitated by ethanol to prepare crude polysaccharide; and the crude polysaccharide is subjected to complex reaction, acid pickling,and deproteinization by a sevag method, and then is purified step by step through graded ethanol precipitation to prepare the target product, namely the D-fraction polysaccharide of the polyporus frondosus. The crude polysaccharide is obtained by a water extraction and ethanol precipitation method, and then is separated and purified according to different natures of a complex compound and the D-fraction polysaccharide to the pH condition. The method has convenient operation, obtains the target product with high purity, and provides a feasible way for producing the D-fraction polysaccharide ofthe polyporus frondosus on a large scale, and preparing various medicaments and health-care foods.

Description

technical field [0001] The invention relates to a method for extracting and separating effective components of edible fungi, in particular to a method for extracting and separating polysaccharide components of Grifola frondosa fruit body or mycelium. Background technique [0002] Grifola frondosa (Polyporus frondosus) belongs to the Phylum Fungi, Basidiomycotina, Phylomycetes, Polyporaceae, and Polyporaceae. Grifola frondosa is a famous edible and medicinal fungus, which is rich in nutrition and delicious. It is a large fungus that is "edible, tonic, and medicinal, and the whole body is a treasure". Grifola frondosa is flat in nature and sweet in taste, and can be used to treat dysuria, edema, beriberi, liver cirrhosis, ascites, diabetes, high blood pressure, obesity and other diseases. [0003] Known to be extracted from the mycelia or fruiting bodies of Grifola frondosa, consisting of a β-1,6-linked glucose backbone with β-1,3-linked glucose branches or with a β- - Polys...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00A23L1/29A23L1/28A23L1/30A23L1/09A23L29/30A23L31/00
Inventor 徐财泉
Owner ZHEJIANG FANGGE PHARMA
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