Method for detecting inserting mutation polymorphism of ox NPM1 gene

Abstract

The invention discloses a method for detecting the inserting mutation polymorphism of an ox NPM1 gene, which comprises the following steps: (1) carrying out PCR amplification by adopting an NPM1 gene-contained ox gene group to be detected as a template and adopting a primer P as a primer; (2) carrying out polyacrylamide gel electrophoresis detection on a fragment after PCR amplification; carryingout electrophoresis for 50-60 minutes with the voltage of 200V and then carrying out EB dyeing; and (3) parting the inserting mutation polymorphism of the NPM1 gene according to a polyacrylamide gel electrophoresis detecting result. The invention provides a method for detecting the polymorphism of the ox NPM1 gene, which is relevant to economic characteristics; aiming at the gene polymorphism withchanged coding protein conformation, which is caused by the inserting mutation of 12bp existing on an NPM1 gene coding area site, after the specific primer PCR amplification is set, the gene type isparted according to the polyacrylamide gel electrophoresis result, thereby providing technical support for detecting the genetic variation of the local ox NPM1 gene in China.

Description

technical field [0001] The invention belongs to the field of molecular genetics and relates to the detection of gene insertion mutation polymorphism, in particular to a 12bp insertion mutation polymorphism of cattle NPM1 gene and a detection method thereof. Background technique [0002] Gene polymorphism refers to the difference in genome sequence between different species or different individuals in the same species. These differences are caused by nucleotide changes in DNA alleles in chromosomes, which include base substitution, insertion, deletion, and repeated sequence copies number of changes. [0003] Single nucleotide polymorphism refers to the polymorphism caused by the replacement of a single nucleotide (A / T / C / G) in the genomic DNA sequence, mainly caused by the conversion or transversion of a single base. SNPs with transformational variants accounted for about 2 / 3, and several other SNPs were at similar levels. The cytosine of the CpG dinucleotide is the most mut...

AI Technical Summary

Problems solved by technology

At present, there is a lack of research in the field of genetic variation of the NPM1 gene in local yellow cattle in China. The functional research of this gene locus and its genetic variation and economic traits (such as: birth weight, body weight, daily gain, body height, body oblique length, chest circumference, ischial end width and other traits) research on the association is still blank

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