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Gene chip for weight control, preparation method, using method and kit of gene chip

A gene chip and weight control technology, applied in the field of genetic testing, to achieve the effects of good specificity, high degree of automation and low price

Inactive Publication Date: 2013-02-06
湖北维达健基因技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] After searching, there is no report on the gene chip for the detection of obesity-related gene polymorphisms.

Method used

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  • Gene chip for weight control, preparation method, using method and kit of gene chip
  • Gene chip for weight control, preparation method, using method and kit of gene chip
  • Gene chip for weight control, preparation method, using method and kit of gene chip

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Experimental program
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Effect test

Embodiment 1

[0052] Preparation of gene chip for body weight control:

[0053] (1) Processing of the chip carrier: the slide glass is washed with chromic acid lotion, soaked overnight, washed with water, soaked in 24wt% ammonia water overnight, washed with water, and then immersed in a 95% (v / v) ethanol solution of aminopropyltrimethoxysilane In the process, adjust the pH value to 4.0 with glacial acetic acid, ultrasonically clean with 95% (v / v) ethanol, dry at 150°C for 4 hours, and formylate with 9% (v / v) glutaraldehyde.

[0054] (2) Preparation and post-processing of the gene chip:

[0055]According to the designed oligonucleotide chip probe sequence, as shown in Table 1, artificially synthesize each oligonucleotide chip probe, and then dilute each oligonucleotide chip probe with sample solution To the final concentration of 50 μM / L, the described sample solution is 750mmol / L sodium chloride, 75mmol / L sodium acetate, 5% (v / v) glycerol, 1wt% polysucrose and 0.wt 1% dodecane Sodium xant...

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Abstract

The invention relates to a gene chip for weight control, a preparation method, a using method and a kit of the gene chip. The gene chip comprises a solid phase carrier, and a specific oligonucleotide probe for gene mononucleotide polymorphic site detection related to fat fixed on the solid phase carrier, and a nucleotide sequence of the oligonucleotide probe is shown in SEQ ID No.1-20. The invention also discloses the preparation method of the gene chip, the using method, and the kit comprising the gene chip. Each related gene polymorphism detection system related to fat is provided. SNPs (Single Nucleotide Polymorphisms) related to a fat gene in a sample to be detected can be rapidly and accurately detected. The related gene polymorphism detection system has high sensitivity, and significance on scientific weight loss to control weight, and can be widely applied to people who want to lose weight. The gene locus selects specific SNPs of Chinese people, and has pertinence to the Chinese people, and a PCR (polymerase chain reaction) primer has good specificity and can better ensure a success rate of an experiment.

Description

technical field [0001] The invention belongs to the field of genetic testing, and in particular relates to a gene chip for body weight control, a preparation method, a use method and a kit thereof. Background technique [0002] The World Health Organization (WHO) has warned that overweight and obesity are the fifth leading risk of death globally. There are 1.1 billion overweight people and 350 million obese people in the world. In 2007, the number of obese people in China exceeded 200 million. [0003] Human obesity is the result of a combination of genes and the environment. Lack of exercise and overeating are the main external factors leading to obesity. However, weight control is not simply eating less and exercising more. Take the same weight loss method of eating less and moving more, some people succeed and others fail. This is mainly due to genetic differences. An individual's genetic information determines the effects of diet and exercise on their body weight. ...

Claims

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Application Information

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IPC IPC(8): C40B40/06C40B50/06C12Q1/68
Inventor 徐谋胜李兵王珊袁雅燕王宁
Owner 湖北维达健基因技术有限公司
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