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Determination of quality features in agricultural and horticultural crops

A horticultural product, a specific technology, applied in the field of determining the quality characteristics of crops and horticultural crops, which can solve the problem of predicting the optimal harvest window without the application of molecular biology technology

Inactive Publication Date: 2010-03-24
表现研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to date, molecular biology techniques have not been applied to predict the expected value of a quality parameter or to determine the optimal harvest window for a given situation.

Method used

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  • Determination of quality features in agricultural and horticultural crops
  • Determination of quality features in agricultural and horticultural crops
  • Determination of quality features in agricultural and horticultural crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] To determine the correlation between gene expression and fruit ripening in a large number of apple and pear cultivars.

[0070] Data were collected for two pear cultivars, Bon Chretien and Forelle, and two apple cultivars, Golden Delicious and Granny Smith, in separate orchards. , the 4 cultivars were harvested in South Africa according to South African conditions (harvesting, short-term storage, conditional delivery to eg European markets). After harvest (within 48 hours), the values ​​of the different markers were determined in the pulp. For this purpose, mRNA was isolated from mixed fruit samples by the CTAB method (Plant Molecular Biology Reporter Vol. 11(2), 1993, pp. 113-116). Different markers were identified by expression profiling techniques described previously and confirmed by RT-PCR. All samples were tested using the primers designed below:

[0071] forward primer reverse primer

[0072] PG GCCCTAATACGGACGGAATTC AATACAGTCATCACCTGTTCCTATAACC

[...

Embodiment 2

[0099] The best time to lift tulips by air was determined by correlation of gene expression around harvest time with flower quality after harvest.

[0100] Three tulip varieties, cultivar 'Apeldoorn', cultivar 'Leen vande Mark' and cultivar 'Prominence' were sampled at the same growing point each time over four years. Sampling was performed at various times in the season, at 2-week intervals 6-7 weeks before the scheduled airlift date, at least 1 week before the confirmed airlift date, at the time of airlift, and 1 week thereafter. Each sampling of the growing point was done in the morning and the sampling treatment was done during the rest of the day. From the outermost bulb piece, take a size of 1cm 2 A piece of tissue was immediately set in liquid nitrogen. Then, in this tissue, the gene expression of a substantial number of genes for the 3 markers identified to be related to the time of harvest was determined in all standard seasons and in each variety.

[0101] To dete...

Embodiment 3

[0121] Determination of the relationship between rose flower quality and gene expression

[0122] Gene expression data were used to estimate vase life of roses. In fact, some roses last only 5 days in a consumer's vase while others (of the same variety) sometimes last as many as 10 days. This is often due to the fact that they are harvested immature in order to compromise on quality.

[0123] The survey found a good correlation between the expression level of a certain gene or a certain gene and the time and quality of harvest. Using the gene expression data, the method mentioned earlier was used to conduct a correlation study with the vase life of roses. Determining the relative expression levels in petals during and after harvest provides an approximate estimate of the expected vase life of roses. The gene used for this purpose is thought to be the GDSL-motif lipase associated with a structural marker, in this case elongation factor 1α, such as Figure 12 shown. Differe...

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Abstract

The present invention relates to a method for predicting the expected value of the quality features in agricultural and horticultural product and a method for predicting the expected optimal time of harvest by comparing expression parameters at the moment prior to harvest or during the postharvest path of genes and / or proteins related to such quality features for that agricultural and horticultural product, with (a) predetermined calibration line(s). The invention also comprises the markers M8, GAST and GDSL motif lipase and the uses thereof, as well as antibodies against them.

Description

technical field [0001] The present invention relates to the determination of markers for quality characteristics and, in connection therewith, the determination of optimal harvest times and / or optimal postharvest routes for agricultural and horticultural products by means of molecular and biological techniques. Background technique [0002] Determining the quality of fresh produce and horticultural products is becoming increasingly important for producers, businesses and consumers. Awareness of the importance of quality has also increased significantly. Strict requirements are placed on the freshness, external characteristics, smell and aroma of edible products. In order to guarantee as much quality as possible, a "track and trace" system has been introduced in the commercial production chain, and the post-harvest route has been optimized to meet these more stringent requirements. Due to constantly changing transport and storage conditions, there is an urgent need for test...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/158
Inventor 安妮·道维·狄波尔迈克尔·约翰尼斯·马库斯·埃毕斯坎普约斯特·约翰尼斯·西奥多勒斯·吉尔肯克伊沃·拉鲁斯
Owner 表现研究有限公司
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