Morphology based method for separating cells

A technology based on morphology and separation method, which is applied in the field of cell single cell image segmentation, can solve the problems of ineffective separation of adherent cells and affect the calculation of cell morphological parameters, etc., and achieve the effect of effective separation
CN101692282AInactive Publication Date: 2010-04-07ZHEJIANG UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
ZHEJIANG UNIV OF TECH
Publication Date
2010-04-07
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention provides a morphology based method for separating cells. The method comprises the following steps: 1) regions are marked, wherein a binary image of a cell image segmentation result is marked based on the morphology to obtain marked value of each adhering region; 2) a monomer cell is extracted, 2.1) a region is extracted; and 2.2) firstly, a marked graph existing adhering regions is found out, a round structure with the size of structure element of 1 is selected and expanded, and expansion is stopped when the boundary of the marked graph exceeds the boundary of an adhering graph, and the size of the marked graph is a whole of the monomer cell in adhering cells; and 3) a reconstructed new binary image set is traversed, attribution of each monomer is determined, and an image set is established according to an coordinate and area information. The method can effectively separate adhering cells, and prepares for accurate calculation on morphological parameters of the cells.
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Description

technical field

[0001] The present invention relates to a cell separation method, in particular to a method for segmenting a single cell according to a cell image. Background technique

[0002] In the medical field, it is usually necessary to segment cells from cell images. Due to the inhomogeneity of gray levels of cell images and the overlapping of spatial positions, the segmentation results include overlapping images of many cells and some over-segmented regions in the nucleus. If the shape is directly calculated without processing, it will be a challenge to the partial attribution of over-segmentation and the accuracy of shape parameter calculation.

[0003] The defects of traditional cell separation methods: 1. It cannot effectively separate adhered cells, and it is impossible to extract single cells from the adhered cells; 2. It affects the accuracy of cell morphological parameter calculation. SUMMARY OF THE INVENTION

[0004] In order to overcome the shortcomings o...

Claims

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