Water-in-oil type nanoemulsion vaccine preparation

Abstract

The invention discloses a water-in-oil type nanoemulsion vaccine preparation, which is prepared from the following raw materials in percentage by weight: 10 to 70 percent of oil, 20 to 60 percent of surfactant, 5 to 30 percent of cosurfactant, 1 to 40 percent of antigen, 0.01 to 40 percent of immunopotentiator, wherein the total weight percentage of the raw materials is 100 percent. The nanoemulsion formulation cannot only improve the stability and safety of the vaccine and reduce side reactions of the vaccine, but also can remarkably improve humoral response and cell response of the antigen and reinforce the immunization of mucous membranes of organisms.

Description

Technical field

[0001] The invention belongs to the technical field of medicine, and specifically relates to a water-in-oil type nanoemulsion vaccine preparation. technical background

[0002] At present, the types of vaccines commonly used at home and abroad are divided into live vaccines and inactivated vaccines, but both have certain limitations in practical applications. Inactivated vaccines stimulate the body to produce cellular immunity and mucosal immunity is weak; attenuated live vaccines are inconvenient to store, have low safety, and are accompanied by vaccination reactions. Therefore, the development of new vaccines that can produce both strong humoral immune antibodies and efficient local mucosal immunity has become the focus of vaccine research. Summary of the invention

[0003] In view of the above-mentioned problems in the prior art, the purpose of the present invention is to provide a convenient and safe preparation of a nanoemulsion vaccine preparation.

[0004] T...

Examples

Embodiment 1

[0020] Example 1: Preparation of Newcastle Disease Polysaccharide Sodium Milk Vaccine

[0021] 1. Preparation of the oil phase: according to the mass ratio of liquid paraffin: Span 80: Tween 80: ethanol=2:1.9:0.9:0.9, weigh the oil phase and stir and mix well;

[0022] 2. Aqueous phase: Dissolve Radix isatidis and Astragalus polysaccharides in a Newcastle disease inactivated virus (Lasota strain) solution to make a mixed solution (Both Radix isatidis and Astragalus polysaccharides account for 1%), as the water phase, the EID before inactivation 50 = 10 -8.83 / 0.1ml;

[0023] 3. The oil phase and the water phase are emulsified and mixed in a mass ratio of 3:1;

[0024] 4. Use a high-pressure homogenizer to perform nano-processing to obtain Newcastle Disease Isatidis and Astragalus polysaccharide nanoemulsion vaccine.

Embodiment 2

[0025] Example 2: Preparation of avian influenza seaweed and epimedium polysaccharide nanoemulsion vaccine

[0026] 1. Preparation of nano-emulsion phase: ethyl acetate: Span 80: Tween 80: propylene glycol = 2:1.9:0.9:0.95 mass ratio, weigh the oil phase and stir and mix evenly;

[0027] 2. Nanoemulsion water phase: add seaweed polysaccharides and epimedium polysaccharides to the avian influenza inactivated virus (H9 subtype) antigen solution to make a mixed solution (seaweed polysaccharides and epimedium polysaccharides both account for 1%), as the water phase , EID before bird flu virus inactivation 50 = 10 -8.38 / 0.1ml;

[0028] 3. Emulsify and mix the water phase and the oil phase according to a mass ratio of 3:1;

[0029] 4. The emulsion is nano-processed with a high-pressure homogenizer to obtain avian influenza algae and epimedium polysaccharide nanoemulsion vaccine.

Embodiment 3

[0030] Example 3: Preparation of Lentinus edodes and Bupleurum polysaccharide nanoemulsion vaccine

[0031] 1. Preparation of nano-emulsified oil phase: According to the mass ratio of isopropyl palmitate: Span 80: Tween 80: absolute ethanol = 2:1.9:0.9:0.95, weigh the oil phase and stir and mix evenly;

[0032] 2. Nanoemulsion water phase: add lentinan and Bupleurum polysaccharide to blue ear disease inactivated antigen (NVDC-JXA strain) to make a mixed solution, as the water phase, the TCD before inactivation 50 = 10 -5.00 / 0.1ml;

[0033] 3. Emulsify and mix the water phase and the oil phase according to a mass ratio of 7:3;

[0034] 4. The emulsion is nano-processed with a high-pressure homogenizer to obtain blue-ear disease mushroom and Bupleurum polysaccharide nanoemulsion vaccine.

Technical field

[0001] The invention belongs to the technical field of medicine, and specifically relates to a water-in-oil type nanoemulsion vaccine preparation. technical background

[0002] At present, the types of vaccines commonly used at home and abroad are divided into live vaccines and inactivated vaccines, but both have certain limitations in practical applications. Inactivated vaccines stimulate the body to produce cellular immunity and mucosal immunity is weak; attenuated live vaccines are inconvenient to store, have low safety, and are accompanied by vaccination reactions. Therefore, the development of new vaccines that can produce both strong humoral immune antibodies and efficient local mucosal immunity has become the focus of vaccine research. Summary of the invention

[0003] In view of the above-mentioned problems in the prior art, the purpose of the present invention is to provide a convenient and safe preparation of a nanoemulsion vaccine preparation.

[0004] T...

Examples

Embodiment 1

[0020] Example 1: Preparation of Newcastle Disease Polysaccharide Sodium Milk Vaccine

[0021] 1. Preparation of the oil phase: according to the mass ratio of liquid paraffin: Span 80: Tween 80: ethanol=2:1.9:0.9:0.9, weigh the oil phase and stir and mix well;

[0022] 2. Aqueous phase: Dissolve Radix isatidis and Astragalus polysaccharides in a Newcastle disease inactivated virus (Lasota strain) solution to make a mixed solution (Both Radix isatidis and Astragalus polysaccharides account for 1%), as the water phase, the EID before inactivation 50 = 10 -8.83 / 0.1ml;

[0023] 3. The oil phase and the water phase are emulsified and mixed in a mass ratio of 3:1;

[0024] 4. Use a high-pressure homogenizer to perform nano-processing to obtain Newcastle Disease Isatidis and Astragalus polysaccharide nanoemulsion vaccine.

Embodiment 2

[0025] Example 2: Preparation of avian influenza seaweed and epimedium polysaccharide nanoemulsion vaccine

[0026] 1. Preparation of nano-emulsion phase: ethyl acetate: Span 80: Tween 80: propylene glycol = 2:1.9:0.9:0.95 mass ratio, weigh the oil phase and stir and mix evenly;

[0027] 2. Nanoemulsion water phase: add seaweed polysaccharides and epimedium polysaccharides to the avian influenza inactivated virus (H9 subtype) antigen solution to make a mixed solution (seaweed polysaccharides and epimedium polysaccharides both account for 1%), as the water phase , EID before bird flu virus inactivation 50 = 10 -8.38 / 0.1ml;

[0028] 3. Emulsify and mix the water phase and the oil phase according to a mass ratio of 3:1;

[0029] 4. The emulsion is nano-processed with a high-pressure homogenizer to obtain avian influenza algae and epimedium polysaccharide nanoemulsion vaccine.

Embodiment 3

[0030] Example 3: Preparation of Lentinus edodes and Bupleurum polysaccharide nanoemulsion vaccine

[0031] 1. Preparation of nano-emulsified oil phase: According to the mass ratio of isopropyl palmitate: Span 80: Tween 80: absolute ethanol = 2:1.9:0.9:0.95, weigh the oil phase and stir and mix evenly;

[0032] 2. Nanoemulsion water phase: add lentinan and Bupleurum polysaccharide to blue ear disease inactivated antigen (NVDC-JXA strain) to make a mixed solution, as the water phase, the TCD before inactivation 50 = 10 -5.00 / 0.1ml;

[0033] 3. Emulsify and mix the water phase and the oil phase according to a mass ratio of 7:3;

[0034] 4. The emulsion is nano-processed with a high-pressure homogenizer to obtain blue-ear disease mushroom and Bupleurum polysaccharide nanoemulsion vaccine.

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