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This invention belongs to the field of plant-derived pesticide technology, specifically relating to a method for preparing a juglone microemulsion and its insecticidal activity. The juglone microemulsion comprises juglone, an oil phase, an emulsifier, a co-emulsifier, and water, with the following mass percentage composition: juglone: ​​2.5–10%, oil phase: 5–25%, emulsifier: 5–15%, co-emulsifier: 5–35%, and the balance being water. The mass ratio of the emulsifier to the co-emulsifier is 1:0.5–1:3. The juglone microemulsion of this invention uses plant-derived juglone as the main active ingredient. The prepared juglone microemulsion is an O / W type microemulsion, a clear, stable brown liquid, which can be diluted and directly sprayed onto the leaves of crops to kill and repel insects.

The invention relates to the technical field of stem cells, in particular to a human umbilical cord mesenchymal stem cell preparation for wound healing and a preparation method thereof. The human umbilical cord mesenchymal stem cell preparation for wound healing comprises human umbilical cord mesenchymal stem cells, a cell antifreezing agent, a cell protective agent, a polymer stabilizer and normal saline, wherein the cell antifreezing agent comprises dimethyl sulfoxide and bioactive polysaccharide. According to the invention, the bioactive polysaccharide is introduced into the cryopreservation solution in the stem cell preparation to partially replace dimethyl sulfoxide, so that the damage of the preparation to cells in the recovery process is obviously reduced; meanwhile, the survival rate and the recovery rate of the recovered cells are improved by utilizing the anti-oxidation and cell membrane protection characteristics of the polysaccharide.