Rapid propagation method of elephant grass

Abstract

The invention relates to a rapid propagation method of elephant grass, which belongs to the technical field of plant cell engineering. Using the young panicle of Elephant Grass as material, the cell-engineered seedlings of Elephant Grass were rapidly propagated by induction of callus, induction of clustered seedlings, multiplication of clustered seedlings, and culture of strong roots; cell-engineered seedlings could be produced from one young panicle by one expansion 85-406 strains, the average propagation coefficient of cell engineering seedlings is 198 strains / ear, and the transplanting survival rate of cell engineering seedlings in greenhouses or field seedbeds reaches 98%. After more than 30 days of growth, they can be used as seedlings for field planting; this method can Improving the established "Elephant grass somatic cell culture plant regeneration method" can not induce clustered seedlings. It is a beneficial supplement to the existing Elephant grass seed stem asexual propagation and seed stem cell engineering propagation technology. It has high reproduction coefficient, convenient material extraction and The characteristics of easy operation provide technical support for the industrial application of elephant grass.

Description

1. Technical field [0001] The invention relates to a rapid propagation method of elephant grass, which belongs to the technical field of plant cell engineering. 2. Background technology [0002] Elephant Grass (Pennisetum Purpureum Schumach), also known as Pennisetum Purpureum Schumach, is a perennial herbaceous plant of the Gramineae Pennisetum genus. It is native to tropical Africa and is suitable for cultivation in tropical and subtropical regions. Elephant grass can not only be used as high-quality green feed for herbivorous livestock and poultry and aquatic fish, ideal raw material for high-quality pulp and medium-to-high-end composite wood-based panels, but also an important biomass energy crop. The planting area at home and abroad is expanding rapidly. [0003] Although Elephant Grass is a perennial herb, it cannot bloom and bear fruit naturally in the middle and lower reaches of the Yangtze River in my country and areas at the same latitude. Propagation usually adopt...

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Problems solved by technology

[0003] Although Elephant Grass is a perennial herb, it cannot bloom and bear fruit naturally in the middle and lower reaches of the Yangtze River in my country and areas at the same latitude. Propagation usually uses stem nodes that have grown for about 2 months as materials for vegetative propagation, which has the disadvantages of low reproduction coefficient and labor-intensive operations.

Jiang Hongru et al. used the young tender stem nodes of Dwarf Elephant Grass as explants to establish the test tube rapid propagation technology system of Dwarf Elephant Grass (such as Jiang Hongru, She Faxin, Wang Biqin, Liu Tengyun. Research on the Test Tube Rapid Propagation Technology of Dwarf Elephant Grass. The full-text database of important conferences in China) has effectively utilized the young stem nodes and has a high reproduction coefficient; Zhong Xiaoxian et al. used the young panicles of Elephant grass as explants to establish the "Plant Regeneration Method of Elephant Grass Somatic Cell Culture" (ZL 200610085256.2), It provides the possibility to use the young panicles of Elephant grass to propagate seedlings, but there are shortcomings that the existing young panicles cannot induce clustered seedlings and the reproduction coefficient is low

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