Method for detecting aconitine in-vitro metabolic product by matrix assisted laser desorption mass spectrum

Abstract

The invention provides a method for detecting an aconitine in-vitro metabolic product by a matrix assisted laser desorption mass spectrum, which comprises the following steps of: adding aconitine into a common anaerobic bacterium culture medium containing the fresh excrement of a healthy rat to culture for a week to obtain culture liquid; extracting the culture liquid with dichloromethane; centrifuging the dichloromethane extract liquid, filtering supernatant liquid by a micropore filtering film and analyzing the aconitine in-vitro metabolic product by the matrix assisted laser desorption mass spectrum. The method analyzes the in-vitro metabolic products of 18 aconitines and is simple, easy, rapid and efficient.

Description

technical field

[0001] The invention relates to a method for detecting metabolites of aconitine in vitro by matrix-assisted laser desorption mass spectrometry. technical background

[0002] Ranunculaceae Aconitum, Aconitum aconitum, Aconitum aconitum and Aconitum aconitum are commonly used Chinese herbal medicines, which have various pharmacological activities such as strengthening the heart, relieving pain and reducing inflammation, and also have certain toxicity. Aconitine is one of the main active ingredients and toxic ingredients. Since traditional Chinese medicine is generally taken orally, its active ingredients will be affected by the intestinal flora in the intestinal tract before reaching the target organ for absorption. Aconitine can be metabolized into monoester alkaloids, diester alkaloids and lipid alkaloids under the action of intestinal flora. (Zhao Yufeng et al., Chemical Journal of Chinese Universities: Vol.28(11), 2007, 2051-2055) Modern pharmacological s...

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