In-vitro cell pressure loading device and in-vitro cell pressure loading method thereof

A loading device and pressure technology, which is applied in the field of tissue engineering and cell engineering, can solve the problems of high price and complex structure, and achieve the effect of simple operation and accurate and reliable pressure effect

Inactive Publication Date: 2010-06-02
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the centrifugal force loading device cannot truly reflect the physiological state of the cells, the Flexercell cell loading device, the Petriperm elastic film culture dish

Method used

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  • In-vitro cell pressure loading device and in-vitro cell pressure loading method thereof
  • In-vitro cell pressure loading device and in-vitro cell pressure loading method thereof
  • In-vitro cell pressure loading device and in-vitro cell pressure loading method thereof

Examples

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Embodiment

[0052] In vitro cell pressure loading devices such as figure 1 As shown, the in vitro cell pressure loading device includes an air pressure source 1, an intake solenoid valve 2, a filter 3, a pressure reducing valve 4, a pressure chamber 5 and a digital control system. The valve is connected with the pressure chamber; one end of the pressure chamber 5 is connected with the air outlet electromagnetic valve 6, and the air outlet electromagnetic valve passes through the drive circuit 7. The multifunctional data acquisition card 8 is connected with the computer 14; the other side of the drive circuit 7 is connected with the intake solenoid valve 2; the other end of the pressure chamber 5 is connected with the pressure sensor 9; the pressure sensor passes through a programmable gain amplifier 10, and the multifunctional data The acquisition card 8 is connected with the computer 14; the pressure chamber 5 is connected with the computer 14 through the standard cylinder 11, the linear...

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Abstract

The invention discloses an in-vitro cell pressure loading device and an in-vitro cell pressure loading method thereof. The in-vitro cell pressure loading device is characterized by comprising a pneumatic source (1), an air inlet electromagnetic valve (2), a filter (3), a decompressing valve (4), a pressure cavity (5) and a digital regulation and control system, wherein the pneumatic source is connected with the pressure cavity by the air inlet electromagnetic valve, the filter and the decompressing valve; one end of the pressure cavity (5) is connected with an air outlet electromagnetic valve (6); the air outlet electromagnetic valve is connected with a computer (14) by a drive circuit (7) and a multifunctional data acquisition card (8); the other side of the drive circuit (7) is connected with the air inlet electromagnetic valve (2); the other end of the pressure cavity (5) is connected with a pressure sensor (9); the pressure sensor is connected with the computer (14) by a programmable gain amplifier (10) and the multifunctional data acquisition card (8); and the pressure cavity (5) is connected with the computer (14) by a standard cylinder (11), a linear stepping motor (12), a power amplifier (13) and the multifunctional data acquisition card (8).

Description

technical field [0001] The invention relates to an in vitro cell pressure loading device and an in vitro cell pressure loading method, belonging to the fields of tissue engineering and cell engineering. Background technique [0002] Cell mechanics is one of the foundations of tissue engineering and cell engineering. It is often necessary to study the influence of mechanical factors on cell morphology and growth. Due to the extremely complex structure of biological tissues and organs, there are also large differences between biological individuals, resulting in complex and diverse mechanical environments in somatic cells, which increases the difficulty of studying the mechanical behavior of somatic cells. Compression of tissues and cells in the body is a common load-bearing method for organisms. For example, in the musculoskeletal system, bones are the main load-bearing structure of the human body. It provides a supporting frame for the human body, protects internal organs, a...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N5/07C12N5/077
Inventor 王军邹远文李晋川黄学进赵志河樊瑜波王娅婷
Owner SICHUAN UNIV
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