In-vitro cell pressure loading device and in-vitro cell pressure loading method thereof

Abstract

The invention discloses an in-vitro cell pressure loading device and an in-vitro cell pressure loading method thereof. The in-vitro cell pressure loading device is characterized by comprising a pneumatic source (1), an air inlet electromagnetic valve (2), a filter (3), a decompressing valve (4), a pressure cavity (5) and a digital regulation and control system, wherein the pneumatic source is connected with the pressure cavity by the air inlet electromagnetic valve, the filter and the decompressing valve; one end of the pressure cavity (5) is connected with an air outlet electromagnetic valve (6); the air outlet electromagnetic valve is connected with a computer (14) by a drive circuit (7) and a multifunctional data acquisition card (8); the other side of the drive circuit (7) is connected with the air inlet electromagnetic valve (2); the other end of the pressure cavity (5) is connected with a pressure sensor (9); the pressure sensor is connected with the computer (14) by a programmable gain amplifier (10) and the multifunctional data acquisition card (8); and the pressure cavity (5) is connected with the computer (14) by a standard cylinder (11), a linear stepping motor (12), a power amplifier (13) and the multifunctional data acquisition card (8).

Description

technical field [0001] The invention relates to an in vitro cell pressure loading device and an in vitro cell pressure loading method, belonging to the fields of tissue engineering and cell engineering. Background technique [0002] Cell mechanics is one of the foundations of tissue engineering and cell engineering. It is often necessary to study the influence of mechanical factors on cell morphology and growth. Due to the extremely complex structure of biological tissues and organs, there are also large differences between biological individuals, resulting in complex and diverse mechanical environments in somatic cells, which increases the difficulty of studying the mechanical behavior of somatic cells. Compression of tissues and cells in the body is a common load-bearing method for organisms. For example, in the musculoskeletal system, bones are the main load-bearing structure of the human body. It provides a supporting frame for the human body, protects internal organs, a...

AI Technical Summary

Problems solved by technology

For example, the centrifugal force loading device cannot truly reflect the physiological state of the cells, the Flexercell cell loading device, the Petriperm elastic film culture dish loading device have complex structures and are expensive, and the fluid shear force loading device is only suitable for in vitro loading experiments on vascular endothelial cells, etc.

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