Method for preparing in-situ therapeutic substance-loaded microcapsules

Abstract

The invention discloses a method for preparing therapeutic substance-loaded microcapsules. The method applies the high-voltage electrostatic spray principle and comprises the steps of leading therapeutic substance-loaded charged polymer solution to instantaneously form ultrafine gel particles under the high-voltage electrostatic action, leading a mist of the particles to be received by polymer solution charged with opposite charges, leading a surface layer of the gel particles to be wrapped by a polymer gel shell layer of the opposite charges, forming structures of capsule cores and capsule walls, further filtering by a microporous membrane, and obtaining the therapeutic substance-loaded microcapsules. Microstructures and scale of the capsule cores and the capsule walls can be precisely controlled through voltage, solution concentration and distance from a jet orifice to receiving solution, and therapeutic substances are bound by the capsule cores and the capsule walls, thereby forming controlled release and improving therapeutic efficacy. The method has the advantages of simple and feasible process and good repeatability, the whole process of the preparation process is clean and pollution-free, the provided in-situ therapeutic substance-loaded microcapsules do not need the complex post-processing, the particle size is controllable, and the release rate of the therapeutic substances is convenient to control, thereby having good application prospect.

Description

technical field [0001] The invention relates to a preparation method of microcapsules, in particular to a preparation method of microcapsules carrying therapeutic substances in situ, and belongs to the technical fields of biomedicine and biomaterials. Background technique [0002] Microcapsules refer to the use of natural or synthetic polymer materials to encapsulate dispersed solids, liquids, and even gaseous substances to form tiny particles with semipermeable or sealed capsule membranes. So far, effective microencapsulation of active substances such as drugs, proteins, growth factors, vitamins, fragrances, antibacterial ions, nanoparticles, etc., and even living cells has been used in many fields such as disease treatment and prevention, functional foods, cosmetics, industrial separation It has become more and more widely used, and has become one of the key research and development high-tech materials in the 21st century. [0003] At present, the preparation methods of m...

AI Technical Summary

Problems solved by technology

Chinese patent CN 100488618C discloses a method for preparing ex-situ drug-carrying liquid core microcapsules by electrostatic spraying. The patent discloses using metal calcium ions as the capsule wall forming agent for alginate microcapsules, although by using malt paste Thickeners such as essence and xanthan gum and calcium ions can lock the alginate on the capsule wall, and after the microcapsules are formed, the wall membrane is strengthened by soaking in calcium chloride solution to form a semipermeable microcapsule wall. However, in the physiological fluid environment, divalent calcium ions are prone to ion exchange with monovalent cations such as potassium and sodium ions, resulting in the rapid collapse of the microcapsule wall membrane layer, and it is difficult to maintain the controlled release and sustained release of drugs

Therefore, such materials are still difficult to meet the application of stable and sustained release of microcapsules

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