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Method for preparing repairing material of antibacterial degradable active nanometer composite bionic tone tissue

A nano-composite and tissue repair technology, applied in the field of biomedical engineering, can solve problems such as multi-animal experiments and clinical trials, and achieve the effects of good environment, high yield and good control.

Inactive Publication Date: 2010-06-09
ANHUI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Continued "trial-and-error" experimentation requires too many animal experiments and clinical trials, which requires almost impossible expensive expenses
Based on the above problems, the medical concept of using biomaterials to replace living tissue to restore functions has been greatly challenged.

Method used

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  • Method for preparing repairing material of antibacterial degradable active nanometer composite bionic tone tissue
  • Method for preparing repairing material of antibacterial degradable active nanometer composite bionic tone tissue
  • Method for preparing repairing material of antibacterial degradable active nanometer composite bionic tone tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation method of the antibacterial and degradable active nanocomposite biomimetic bone tissue repair material is characterized in that it includes the following steps:

[0034] A. Dissolve 8g of conventional hydroxyapatite (HA) in 20ml of 5M hydrochloric acid solution, and mix with 10ml of 2% minocycline in 0.1M hydrochloric acid solution into 100ml of 2% gelatin aqueous solution.

[0035] B. Keep the above mixed solution obtained in a silicone oil reaction bath at 40°C under continuous stirring conditions. Adjust the pH value of the reaction system to 7-8 with 1M NaOH, and react for 2 hours.

[0036] C. After the reaction is complete, adjust the pH value of the reaction system to 7 dropwise with 0.1M hydrochloric acid and sodium hydroxide and keep it stable. Then stand at room temperature and age for 24 hours.

[0037] D. The final reaction product is centrifuged, and the supernatant is discarded; the precipitate is washed with deionized water, centrifuged, ...

Embodiment 2

[0044] A, 9.9721g of calcium chloride (CaCl 2 ) and 5.7309g of sodium dihydrogen phosphate (NaH2 PO 4 ) was dissolved in 20ml of 1M hydrochloric acid solution. Dissolve 0.2 g of minocycline in 10 ml of 0.1 M hydrochloric acid solution; then mix the prepared calcium / phosphorus solution and minocycline solution into 100 ml of 2% gelatin aqueous solution respectively.

[0045] B. Keep the above mixed solution obtained in a silicone oil reaction bath at 40°C under continuous stirring conditions. Adjust the pH value of the reaction system to 7-8 with 1M NaOH, and react for 2 hours.

[0046] C. After the reaction is complete, adjust the pH value of the reaction system to 7 dropwise with 0.1M hydrochloric acid and sodium hydroxide and keep it stable. Then stand at room temperature and age for 24 hours.

[0047] D. The final reaction product is centrifuged, and the supernatant is discarded; the precipitate is washed with deionized water, centrifuged, and the operation is repeated ...

Embodiment 3

[0049] A. Combine 4.51481g of calcium hydroxide (CaOH) and 5.7309g of sodium dihydrogen phosphate (NaH 2 PO 4 ) was dissolved in 20ml of 1M hydrochloric acid solution. Dissolve 0.2 g of minocycline in 10 ml of 0.1 M hydrochloric acid solution; then mix the prepared calcium / phosphorus solution and minocycline solution into 100 ml of 2% gelatin aqueous solution respectively.

[0050] B. Keep the above mixed solution obtained in a silicone oil reaction bath at 40°C under continuous stirring conditions. Adjust the pH value of the reaction system to 7-8 with 1M NaOH, and react for 2 hours.

[0051] C. After the reaction is complete, adjust the pH value of the reaction system to 7 dropwise with 0.1M hydrochloric acid and sodium hydroxide and keep it stable. Then stand at room temperature and age for 24 hours.

[0052] D. The final reaction product is centrifuged, and the supernatant is discarded; the precipitate is washed with deionized water, centrifuged, and the operation is r...

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Abstract

The invention relates to a method for preparing repairing material of antibacterial degradable active nanometer composite bionic tone tissue, comprising the following steps of: A, preparing a calcium-phosphorus solution with calcium chloride, sodium dihydrogen phosphate and the like, and mixing the calcium-phosphorus solution, the minocycline solution and the aqueous solution of gelation; B, carrying out reaction on the mixed solution in a silicone oil reactive bath; C, standing in motionless and ageing at the room temperature; D, centrifugalizing the aged substance to obtain the precipitate;freezing and drying the precipitate to obtain the repairing material for the bionic nanometer composite bionic bone tissue. The method adopts molecular bionic synthesis technology, simulates the process of bone mineralization and takes gelatine with excellent biocompatibility as an organic molecule template that regulates and controls growth of apatite (primary inorganic component of bone tissue)under physiologic condition to carry out biomimetic synthesis of repairing material for organic-inorganic composite bone tissue. Meanwhile, the antibacterial agent minocycline in the process of the growth of apatite crystal is loaded. Therefore, a novel antibacterial repairing material of nanometer composite bionic tone tissue is synthesized.

Description

technical field [0001] The invention belongs to biomedical engineering technology, and relates to a preparation method for bone defect repair materials used in orthopedics, extracerebral and oral cavity clinics. Background technique [0002] The repair of human hard tissue defects caused by inflammation, trauma, tumors and the aging of society is a difficult problem to be solved in stomatology and clinical medicine. According to reports, the current annual turnover of biomaterials in the world is 12 billion US dollars, of which the human body hard-woven replacement materials are about 2.3 billion US dollars. And it is growing at a rate of 7%-12% per year. According to the report of the civil affairs department, there are about 8 million disabled patients in my country at present, and 3 million patients with bone defects in the whole country every year, and 20-33% of the total number of patients with tooth defects. Therefore, materials for repairing human hard tissue defects...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/54A61L27/58A61K6/033A61K6/02A61K6/838
Inventor 李全利周健窦晓晨蔡华琼汤键周剑沈军
Owner ANHUI MEDICAL UNIV
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