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Arbuscular mycorrhizal phytoremediation method for polycyclic aromatic hydrocarbon polluted soil

A technology for polycyclic aromatic hydrocarbons and polluted soil, applied in the field of contaminated soil remediation, can solve the problems of no economical, efficient, and practical in-situ remediation technology, and achieve low cost, simple technical process, and good remediation effect

Inactive Publication Date: 2010-06-09
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Remediation of soil contaminated by organic non-point sources such as PAHs is one of the technical issues of common concern in the field of soil and environment at home and abroad; but so far there are almost no economical, efficient and practical in-situ remediation technologies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Contains phenanthrene 59.8mg·kg -1 The contaminated soil was ground and passed through a 3mm sieve and placed in a series of pots with a volume of 500g. Each pot contained 200g of contaminated soil, and Glomus mosseae (BGC JX01) and Glomus etunicatum were added respectively. , BGC SC01C), Glomus versiforme (BGC USA03), Glomus constrictum (BGC USA02) and Glomus intraradices (BGCUSA04) each 20g, and then cover 150g of pollution Soil, add water to 50% water content, let stand overnight. Alfalfa (Medicagosativa L.) seeds with 10% H 2 o 2 Soak the seeds for 10 minutes, and sow them in pots and bowls after germination. After the plants emerge, they are managed according to the management methods of common crops; when the plants grow to about 10 cm in height, 6 seedlings are reserved for each pot. After 60 days, soil samples were collected to analyze the removal rate of phenanthrene in the soil and evaluate the restoration effect.

[0014] After 60 days, the removal rates...

Embodiment 2

[0016] Contains 103mg·kg -1 Phenylhex 74mg·kg -1 The composite contaminated soil of pyrene was ground and passed through a 3mm sieve and placed in a series of pots with a volume of 500g. Each pot was filled with 200g of contaminated soil, and 20g of Glomus mosseae (BGCHUB01A) bacterial agent was added respectively, and then covered with 150g. Contaminated soil, add water to 50% water content, let stand overnight. Alfalfa (Medicagosativa L.) seeds with 10% H 2 o 2 Soak the seeds for 10 minutes, and sow them in pots and bowls after germination. After the plants emerge, they are managed according to the management methods of common crops; when the plants grow to about 10 cm in height, 6 seedlings are reserved for each pot. After 70 days, soil samples were collected to analyze the removal rate of phenanthrene and pyrene in the soil and evaluate the restoration effect. After 70 days, the removal rates of phenanthrene and pyrene in the soil were 99% and 88%, respectively.

Embodiment 3

[0018] Contains 103mg·kg -1 Phenyl or 74mg·kg -1 Pyrene (single) contaminated soil was ground and passed through a 3mm sieve and placed in a series of pots with a volume of 500g. Each pot was filled with 200g of contaminated soil, and Glomus mosseae (BGC HUB01A) and young capsules were added Mold (Glomus etunicatum, BGC HEB04) (composite) bacterial agent 20g, then cover 150g of contaminated soil, add water to 50% water content, and let stand overnight. Alfalfa (Medicagosativa L.) seeds with 10% H 2 o 2 Soak the seeds for 10 minutes, and sow them in pots and bowls after germination. After the plants emerge, they are managed according to the management methods of common crops; when the plants grow to about 10 cm in height, 6 seedlings are reserved for each pot. After 70 days, soil samples were collected to analyze the removal rate of phenanthrene and pyrene in the soil and evaluate the restoration effect. After 70 days, the removal rates of phenanthrene or pyrene from the s...

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Abstract

The invention discloses an arbuscular mycorrhizal phytoremediation (AMPR) method for polycyclic aromatic hydrocarbon polluted soil, and belongs to the technical field of polluted soil remediation. The method comprises the following steps: (1) inoculating glomus mosseae and other arbuscular mycorrhizal fungal agents to a surface soil layer (0 to 20cm) of the polycyclic aromatic hydrocarbon polluted soil; and (2) planting Medicagosativa L. and other leguminous plants, harvesting the plants after the plants grow for 60 to 80 days, collectively burning the plants after drying and transferring. The method has the advantages that the adopted arbuscular mycorrhizal fungus can easily symbiose with terrestrial plants, promotes the number and activity of rhizospheric microorganisms, and improves the degradation efficiency of PAHs; and the method has simple technical process, low cost, aesthetic property and safety and good remediation effect on the PAHs and other organic polluted soil, and is applicable to treatment of area source organic polluted soil.

Description

technical field [0001] The invention relates to the technical field of contaminated soil remediation, in particular to a method for arbuscular mycorrhizal remediation (AMPR, Arbuscular mycorrhizal phytoremediation) polycyclic aromatic hydrocarbon contaminated soil. Background technique [0002] Soil is one of the most important natural resources on which human beings depend. But at present, there are serious soil pollution problems in our country and many countries or regions in the world. Pollution of toxic organic substances in soil is more prevalent than other pollutants. Among them, most of the polycyclic aromatic hydrocarbons (PAHs) have "three effects" and are easy to persist in the soil, posing a serious threat to human health. Remediation of soil contaminated by organic non-point sources such as PAHs is one of the technical issues of common concern in the field of soil and environment at home and abroad; but so far there are almost no economical, efficient and prac...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10
Inventor 高彦征李秋玲
Owner NANJING AGRICULTURAL UNIVERSITY
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