Method for constructing expression system of exogenous genes in campylobacter jejuni and shuttle plasmids thereof

Abstract

The invention relates to a method for constructing an expression system of exogenous genes in a campylobacter jejuni. Under the condition of anaerobic environment and temperature controlled at 37 DEG C, the method comprises the steps of preparing promoters, constructing the shuttle plasmids, and establishing a bioluminescent transgene model of the campylobacter jejuni. By using electrotransformation to introduce the campylobacter jejuni ATCC33291 and ATCC35921 which are expressed under the selection pressure of kanamycin antibiotics, the method can observe bioluminescence without adding any substrate, visually expresses bioinformation, and has a wide research prospect.

Description

technical field

[0001] The invention relates to a construction method of an exogenous gene expression system and a shuttle plasmid thereof, in particular to a construction method of an exogenous gene (Lux) expression system in Campylobacter jejuni and a shuttle plasmid thereof. Background technique

[0002] In recent years, gene recombination technology has developed rapidly. People have been able to link genes with certain functions to appropriate vectors and transform them into bacteria, plants, and animal cells by chemical or physical methods. This technology has been applied It has broad application prospects in the manufacture of exogenous protein products, organ transplantation, animal and plant transgenic breeding, gene therapy, and biosensors.

[0003] Nowadays, people use exogenous gene expression technology to produce biosensors. Different from traditional detection methods, the new biosensors are to directly inject a foreign aid gene (DNA or RNA) encoding a certai...

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