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Method for preparing casein peptide chelated iron

A technology of casein peptide and chelated iron is applied in the field of preparation of casein peptide chelated iron, which can solve the problems of difficult absorption, weak bonding strength and the like, and achieves the effects of low cost and simple process

Inactive Publication Date: 2010-06-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But larger molecular size results in weaker bonds and more difficult absorption

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) First, 400 g of casein was dissolved in 2 L of deionized water to make a casein suspension; then the pH of the casein suspension was adjusted to 13 with NaOH.

[0016] (2) Add 8 g of alkaline protease to the casein suspension to obtain an enzymolysis solution, the enzymolysis temperature is 55° C., and the enzymolysis time is 5 hours;

[0017] (3) Filtrate the enzymatic solution to filter out impurities; add H 2 SO 4 Adjust the pH value of the enzymolysis solution to 5 to obtain a spare enzymolysis solution.

[0018] (4) Add deionized water into the reactor, the amount added is 3 times the volume of the spare enzymolysis solution, heat to 50°C, then add 1 / 3 of the spare enzymolysis solution into the deionized water, stir well and then add FeSO 4 , adding FeSO 4 The amount is 10% of the weight of the standby enzymolysis solution added, and it is incubated and stirred at 50°C for 1 hour to carry out the chelation reaction to obtain the primary chelation solution. ...

Embodiment 2

[0022] (1) First, 600 g of casein was dissolved in 1.2 L of deionized water to make a casein suspension; then KOH was used to adjust the pH of the casein suspension to 7.

[0023] (2) Add 20 g of trypsin to the casein suspension to obtain an enzymolysis solution, the enzymolysis temperature is 65° C., and the enzymolysis time is 1 hour;

[0024] (3) Filtrating the enzymolysis solution to remove impurities; adding HCl to adjust the pH value of the enzymolysis solution to 2 to obtain a spare enzymolysis solution.

[0025] (4) Add deionized water into the reactor, the amount added is 4 times the volume of the spare enzymolysis solution, heat to 30°C, then add 2 / 3 of the spare enzymolysis solution into the deionized water, stir well and then add FeCl 2 , adding FeCl 2 The amount is 5% of the weight of the standby enzymolysis solution added, and it is incubated and stirred at 30°C for 0.5 hour to carry out the chelation reaction to obtain the primary chelation solution.

[0026]...

Embodiment 3

[0029] (1) First, dissolve 300g casein in 6L deionized water to make a casein suspension; then use NH 3 .H 2 O adjusts the pH value of the casein suspension to 10.

[0030] (2) Add 2 g of alkaline protease and 1 g of trypsin to the casein suspension to obtain an enzymolysis solution, the enzymolysis temperature is 45° C., and the enzymolysis time is 10 hours;

[0031] (3) Filtrating the enzymolysis solution to remove impurities; adding HCl to adjust the pH value of the enzymolysis solution to 7 to obtain a spare enzymolysis solution.

[0032] (4) Add distilled water into the reactor, the amount added is 5 times the volume of the spare enzymolysis solution, heat to 65°C, then add 1 / 2 of the spare enzymolysis solution to the distilled water, stir well and then add FeCl 2 , adding FeCl 2 The amount is 40% of the weight of the standby enzymolysis solution added, and it is incubated and stirred at 65° C. for 2 hours to carry out the chelation reaction to obtain the primary chela...

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PUM

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Abstract

The invention relates to a method for preparing casein peptide chelated iron. The method comprises the following specific steps: firstly dissolving the casein into deionized water to prepare casein suspension; then adding protease into the casein suspension to obtain enzymolysis liquid; adding acid into the filtered enzymolysis liquid and regulating the pH value from 2 to 7; heating the deionized water or distilled water, then adding 1 / 3-2 / 3 of enzymolysis liquid into the deionized water or distilled water and adding ferrous sulfate or ferrous chloride; adding the residual standby enzymolysis liquid and adding the ferrous sulfate or ferrous chloride to carry out chelation reaction; and obtaining the casein peptide chelated iron after drying and purifying the reaction liquid. The obtained product has higher purity and total yield and is simple and convenient in technology, low in cost and suitable for scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of additives, and in particular relates to a preparation method of casein peptide chelated iron used as nutritional food in the technical field of animals. Background technique [0002] A chelate is a compound that chelates organic molecules (amino acids or peptide chains) and metal ions through strong coordination bonds. These compounds are widely used in the field of zootechnology because their effectiveness derives from the biological activity of the metals involved, which act as activators in many enzymatic reactions and as regulators in various metabolic functions in all living organisms. [0003] Metal ions, due to the chelation of bonds with peptide chains or amino acid substances, facilitate the absorption, utilization and use of metal ions because they are transported to all regions of the organism through organic components. Especially in the field of animal technology, these compounds are used in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/304A23L1/305A23L33/165A23L33/18
Inventor 冯杰王勇周源马文强
Owner ZHEJIANG UNIV
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