Mushroom polysaccharide chitosan nanoparticle and preparation method thereof

A technology of chitosan nanoparticles and lentinan, which is applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problems of difficult preparation and unfavorable encapsulation, and achieve encapsulation High efficiency and high stability

Inactive Publication Date: 2010-06-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention provides a nano-particle of lentinan and chitosan, which is used to make up the blank of the existing nano-preparation of lentinan, which adopts chitosan and sodium tripolyphosphate as carrier materials, and carries the lentinan by ion gelation

Method used

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  • Mushroom polysaccharide chitosan nanoparticle and preparation method thereof
  • Mushroom polysaccharide chitosan nanoparticle and preparation method thereof
  • Mushroom polysaccharide chitosan nanoparticle and preparation method thereof

Examples

Experimental program
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Example Embodiment

Example 1

(1) 250 mg of chitosan with a molecular weight of 50kDa and a degree of deacetylation of 95% was dissolved in an aqueous acetic acid solution with a mass percentage of 1%, and magnetically stirred at room temperature for 10 minutes at a stirring speed of 70r / min to make the chitosan in Swell in an acetic acid environment and prepare a chitosan solution with a chitosan concentration of 1.5g / L;

(2) Hot-dissolve 6 mg of lentinan in 100 ml of water to prepare a lentinan solution with a concentration of 60 μg / mL;

(3) Accurately measure 10ml of lentinan solution and add it to the above chitosan solution, adjust the pH of the chitosan solution to 5 with a 1% mass percentage concentration of NaOH aqueous solution, and then slowly add 4.5ml mass percentage concentration It is a 1% sodium tripolyphosphate aqueous solution (the weight of sodium tripolyphosphate is 45 mg), so that sodium tripolyphosphate and chitosan can spontaneously form drug-loaded nanoparticles through anion...

Example Embodiment

Example 2

(1) 250 mg of chitosan with a molecular weight of 10kDa and a degree of deacetylation of 90% was dissolved in an aqueous acetic acid solution with a mass percentage of 0.5%, and magnetically stirred at room temperature for 10 minutes at a stirring speed of 70r / min to make the chitosan in Swell in an acetic acid environment and prepare a chitosan solution with a chitosan concentration of 2g / L;

(2) Hot-dissolve 6 mg of lentinan in 100 ml of water to prepare a lentinan solution with a concentration of 60 μg / mL;

(3) Accurately measure 10ml of lentinan solution and add it to the above chitosan solution, adjust the pH of the chitosan solution to 5 with a 1% mass percentage concentration of NaOH aqueous solution, and then slowly add 4.5ml mass percentage concentration It is a 1% sodium tripolyphosphate aqueous solution (the weight of sodium tripolyphosphate is 45 mg), so that sodium tripolyphosphate and chitosan can spontaneously form drug-loaded nanoparticles through anion...

Example Embodiment

Example 3

(1) 250 mg of chitosan with a molecular weight of 50kDa and a degree of deacetylation of 90% was dissolved in an aqueous solution of acetic acid with a mass percentage of 2.5%, and magnetically stirred at room temperature for 10 minutes at a stirring speed of 70r / min to make the chitosan in Swell in an acetic acid environment and prepare a chitosan solution with a chitosan concentration of 2.5g / L;

(2) Hot-dissolve 6 mg of lentinan in 100 ml of water to prepare a lentinan solution with a concentration of 60 μg / mL;

(3) Accurately measure 10ml of lentinan solution and add it to the above chitosan solution, adjust the pH of the chitosan solution to 5 with a 1% mass percentage concentration of NaOH aqueous solution, and then slowly add 4.5ml mass percentage concentration It is a 1% sodium tripolyphosphate aqueous solution (the weight of sodium tripolyphosphate is 45 mg), so that sodium tripolyphosphate and chitosan can spontaneously form drug-loaded nanoparticles through ...

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Abstract

The invention discloses a mushroom polysaccharide chitosan nanoparticle and a preparation method thereof, and the mushroom polysaccharide chitosan nanoparticle is prepared by the following parts by weight of pharmaceutical raw materials and carrier material: 150-250 parts of chitosan, 30-70 parts of sodium tripolyphosphate and 0.6-1.5 parts of mushroom polysaccharide. The chitosan is adopted as a carrier, a drug-loaded nanoparticle is self-formed through the electrostatic action of anions and cations of the chitosan and the sodium tripolyphosphate, and the mushroom polysaccharide chitosan nanoparticle with high stability, good encapsulation efficiency and high drug loading capacity is successfully prepared by combining the ion gelatinization method with the double emulsion-solvent evaporation method for loading water soluble and macromolecular mushroom polysaccharide. The mushroom polysaccharide chitosan nanoparticle has good anti-tumor effect and immune enhancement effect; compared with mushroom polysaccharide solution, the mushroom polysaccharide chitosan nanoparticle has stronger anti-tumor effect and higher stability. The preparation method has the advantages of simple operation, safety and effectiveness and is in line with the environmental protection requirement and applicable to industrial production.

Description

technical field The invention relates to the field of pharmaceutical nano preparations, in particular to a lentinan chitosan nanoparticle and a preparation method thereof. Background technique Polysaccharides are formed by the condensation and dehydration of multiple monosaccharide molecules. There are various types and complex structures, and they are widely distributed in animals and plants. At present, there are many studies on the physical and chemical properties and biological functions of polysaccharides. In recent years, some researchers have carried out further research and development on the potential functions of polysaccharides from different sources, which has attracted wide attention in the application of polysaccharides in the field of medicine. At present, an important application field of polysaccharides is anti-tumor. Through research, it is found that the anti-tumor effects of polysaccharides are as follows: on the one hand, they exert anti-tumor effects b...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K31/715A61K47/36A61P1/16A61P31/20A61P35/00
Inventor 高建青李旎
Owner ZHEJIANG UNIV
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