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Protein relevant to retina photoreaction as well as coding gene and applications thereof

A retina, retinal disease technology, applied in anti-animal/human immunoglobulin, application, gene therapy, etc.

Inactive Publication Date: 2012-03-28
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that GARP protein is involved in the regulation of light-sensing signal transmission in rod cells, but there are still many gaps in the research on its regulation mechanism and mechanism of action (Pentia DC, 2006, J Biol Chem, 281: 5500-5505)

Method used

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  • Protein relevant to retina photoreaction as well as coding gene and applications thereof
  • Protein relevant to retina photoreaction as well as coding gene and applications thereof
  • Protein relevant to retina photoreaction as well as coding gene and applications thereof

Examples

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Embodiment 1

[0039] Example 1. Protein MGARP related to retinal light response

[0040] 1. Acquisition of the MGARP gene

[0041] The mouse retina-specifically expressed gene fragments screened by gene chip technology were used as templates, and the sequence results obtained by sequencing were searched by NCBI BLASTn tool (http: / / www.ncbi.nih.gov / BLAST / ) to obtain possible cDNA And EST sequence fragments were used as reference sequences, primers MGARP1 and MGARP2 were designed, and the complete coding sequence of the mouse was cloned.

[0042] MGARP1: 5'-CGA GAA TTC ACC ATG TAT CTC CGC AGG GCT GTG;

[0043] MGARP2: 5'-CCG CTC GAG ACC TTG GGG TGA TGC TGT TTC.

[0044] Using the total retinal RNA extracted from Kunming mice (Beijing Experimental Animal Research Center) as a template, the target fragment was amplified with the One Step RNA PCR Kit (AMV) from TaKaRa Company.

[0045] The PCR amplification conditions were as follows: first 94°C for 4min; then 94°C for 45sec, 60°C for 45sec, ...

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Abstract

The invention discloses a protein relevant to retina photoreaction as well as coding gene and applications thereof. The protein is named as MGARP, which is the protein of the following a) or b); wherein a) the amino acid sequence of a) is the sequence 2 in sequence table; and b) is the protein derived from a) through replacing and / or deleting and / or adding one or a plurality of amino acid in the amino acid sequence limited by the sequence 2 in the sequence table and relevant to retina photoreaction. The invention also discloses the coding gene of MGARP protein. The MGARP gene can be used for establishing a transgenic mouse model or drug screening model. The MGARP gene or MGARP protein can also be used for screening or preparing drugs used for curing retinal diseases or drugs used for curing energy metabolism diseases.

Description

technical field [0001] The invention relates to a protein related to retinal light response, its coding gene and application. Background technique [0002] The retina is an important part of the animal eye wall tissue and an essential signal conversion area in the light-sensing signal pathway, which converts the external light-sensing signal into a nerve signal and transmits it to the brain through the optic nerve. The occurrence of retinopathy often leads to loss of vision or even blindness, among which age-related macular degeneration (Age-related Macular Degeneration, AMD) and diabetic retinopathy (Diabetic Retinopathy, DR) are typical retinopathy diseases. In recent years, the incidence of retinopathy has increased year by year, among which AMD has become the main blinding disease of the elderly, and the incidence rate of people over 70 years old is close to 20% (Marx J et al., 2006, Science, 311: 1704-1705; Hollyfield JG et al., 2008, Nature Medicine, 14: 194-198). DR...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11C07K16/18A01K67/027C12Q1/68G01N33/68A61K38/17A61K48/00A61P27/02
Inventor 张淑平戚少玲王倚峰张少民
Owner TSINGHUA UNIV