Micro-fluidic chip and method for research on oriented movement of cell in three-dimensional medium

A microfluidic chip, directional movement technology, applied in tissue cell/virus culture devices, microorganism measurement/inspection, biochemical equipment and methods, etc. simple effect

Inactive Publication Date: 2010-06-23
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
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Problems solved by technology

The traditional models used to study cell migration in vitro can only observe the crawling of cells on a two-dimensional surface, which is quite different from the three-dimensional space in which cells live in vivo.

Method used

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  • Micro-fluidic chip and method for research on oriented movement of cell in three-dimensional medium
  • Micro-fluidic chip and method for research on oriented movement of cell in three-dimensional medium
  • Micro-fluidic chip and method for research on oriented movement of cell in three-dimensional medium

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Embodiment

[0021] The microfluidic chip used was designed and manufactured by our laboratory. The chip material is PDMS polymer, which is sealed to the glass surface by irreversible sealing technology. Such as figure 1 As shown, the dimensions of the two parallel perfusion channels are 5 mm in length and 400 μm in width, and the size of the oval cell culture pool is 1 mm in long diameter and 200 μm in short diameter. Add the mixture of breast cancer cells MCF7 and Basement Membrane Extract (BME) into the cell culture tank through the cell inlet at low temperature. After the mixture forms a gel, the upper ends of the perfusion channels on both sides are connected to syringe pumps, and the cells are perfused in the perfusion channels. culture medium at 37°C in CO 2 Cells were incubated in an incubator for 24 hours. Rodamin-123 (Rh-123) and PI were used to jointly detect the activity of MCF7 cells embedded in BME, and the results showed that the survival rate of MCF7 cells in BME for 24 ...

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Abstract

The invention provides a micro-fluidic chip and a method for research on oriented movement of cell in three-dimensional medium. The micro-fluidic chip comprises a cell injection port, a cell culture pool, two parallel perfusion channels and a waste liquid pool; the lower ends of the perfusion channels are connected with the waste liquid pool, the cell culture pool is connected with the two parallel perfusion channels, the upper end of the waste liquid pool is connected with the cell injection port; and the micro-fluidic chip is used to provide three-dimensional space support for growth and migration of the cell, simultaneously stable concentration gradient is formed in the three-dimensional space, and with the inducement of the chemoattractant, the cell moves in orientation in the three-dimensional medium. The invention has the advantages of simple and fast operation, low sample use level and the like.

Description

technical field [0001] The present invention relates to the application of microfluidic chip technology to the field of biomedical research, provides a microfluidic chip and a method for studying directional movement of cells in a three-dimensional medium, and uses the microfluidic chip to provide three-dimensional space support for cell growth and migration , and generate a stable concentration gradient in this three-dimensional space. Under the induction of chemoattractants, cells move directional in the three-dimensional medium, and the movement of two different cells under the same conditions can be investigated simultaneously. Background technique [0002] Directed cell movement plays an important role in embryonic development, wound healing, tumor invasion and metastasis. The traditional models used to study cell migration in vitro can only observe the crawling of cells on a two-dimensional surface, which is quite different from the three-dimensional space in which cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/00C12Q1/02
Inventor 秦建华刘婷姣林炳承
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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