New process for extracting root of largeleaf gentian

A Gentiana and solvent technology, applied in the field of extracting gentiopicroside, can solve the problems of easy loss of samples and cumbersome steps, and achieve the effect of high product purity and simple separation steps

Inactive Publication Date: 2010-06-30
NANTONG XINYING DESIGN SERVICE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the method of extracting gentiopicroside from Gentiana is mainly column chromatography, which mainly uses solid packing, which is prone to irreversible adsorption, has the disadvantages of easy loss of samples, and cumbersome steps.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Analytical high-speed countercurrent chromatography is applied, and the solvent system adopts chloroform-methanol-n-butanol-water. The solvent chloroform:methanol:n-butanol:water was placed in a separatory funnel at a volume ratio of 6:5:4:5, shaken up and allowed to stand to separate layers. After equilibrating for a period of time, separate the upper and lower phases, the upper phase is the stationary phase, and the lower phase is the mobile phase. Dissolve an appropriate amount of Gentiana quinquefolium crude extract in 2ml of the lower phase for use. Using the CCC-3000 high-speed countercurrent chromatograph made in the United States, fill the chromatographic separation column with the stationary phase, turn on the speed controller, and start to pump the mobile phase at a certain flow rate when the predetermined speed is reached. When the mobile phase flows out, it passes through the six-way The injection valve injects the sample. Elute from the head end to the tai...

Embodiment 2

[0011] Analytical high-speed countercurrent chromatography is applied, and the solvent system adopts chloroform-methanol-n-butanol-water. The solvent chloroform:methanol:n-butanol:water was placed in a separatory funnel at a volume ratio of 5:4:3:4, shaken evenly and allowed to stand to separate layers. After equilibrating for a period of time, separate the upper and lower phases, the upper phase is the stationary phase, and the lower phase is the mobile phase. Dissolve an appropriate amount of Gentiana quinquefolium crude extract in 2ml of the lower phase for use. Using the CCC-3000 high-speed countercurrent chromatograph made in the United States, fill the chromatographic separation column with the stationary phase, turn on the speed controller, and start to pump the mobile phase at a certain flow rate when the predetermined speed is reached. When the mobile phase flows out, it passes through the six-way The injection valve injects the sample. Elute from the head end to the...

Embodiment 3

[0013] Analytical high-speed countercurrent chromatography is applied, and the solvent system adopts chloroform-methanol-n-butanol-water. The solvent chloroform:methanol:n-butanol:water was placed in a separatory funnel at a volume ratio of 4:5:5:4, shaken up and allowed to stand to separate layers. After equilibrating for a period of time, separate the upper and lower phases, the upper phase is the stationary phase, and the lower phase is the mobile phase. Dissolve an appropriate amount of Gentiana quinquefolium crude extract in 2ml of the lower phase for use. Using the CCC-3000 high-speed countercurrent chromatograph made in the United States, fill the chromatographic separation column with the stationary phase, turn on the speed controller, and start to pump the mobile phase at a certain flow rate when the predetermined speed is reached. When the mobile phase flows out, it passes through the six-way The injection valve injects the sample. Elute from the head end to the tai...

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Abstract

The invention relates to a method for extracting gentiamarin from root of largeleaf gentian by using high-speed countercurrent chromatography. Two mutually-immiscible solvent systems are utilized to perform the separation and purification on natural products in a mode of high-speed planetary motion in a support pipe, and thus the two solvent systems have no irreversible adsorption. The method is simple in separation steps and the prepared product has high purity.

Description

Technical field: [0001] The invention relates to a method for extracting gentiopicroside from Gentiana. In particular, a method for extracting gentiopicroside from Gentiana by high-speed countercurrent chromatography. Background technique: [0002] Gentiana is a commonly used traditional Chinese medicine, which is derived from the drying of Gentiana macrophylla Pall., G.st raminea Maxim., G. crassicaulis Duthie ex Burk, or G. dahurica Fisch. Roots, the first three are commonly called "Gentica qin" and "Genida mahua" respectively according to their different characters, and the latter is commonly called "Gentica chinensis". It tastes bitter, pungent, slightly cold, and mainly contains alkaloids, iridoid glycosides, sterol glycosides and other components. Gentiana has the effects of sedation, analgesia, antipyretic and inhibition of reflex intestinal secretion. Gentiana chinensis is widely used clinically in the treatment of rheumatism. [0003] Gentiana chinensis is mainl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08A61K36/51
Inventor 张炎
Owner NANTONG XINYING DESIGN SERVICE
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