Method for preparing immunogen chloramphenicol

Abstract

The invention discloses a method for preparing immunogen chloramphenicol, which is characterized by comprising the following main steps: dissolving bovine serum albumin, chloramphenicol sodium succinate and carbodiimide in phosphate buffer solution in a molar ratio of 1:50:1,200 respectively and mixing the three kinds of solution to perform a reaction at room temperature for 2 hours while slowly shaking for making the solution react fully; filling liquid obtained after reaction in a dialysis bag, placing the dialysis bag in one liter of phosphate buffer solution to perform dialysis for 48 hours and changing the solution every eight hours; and collecting the liquid in the dialysis bag, pre-freezing the liquid at 20 DEG C below zero for 2 hour and subjecting the product of the pre-freezing to freeze drying at 50 DEG C below zero to obtain white solid powder of immunogen chloramphenicol. The preparation method of the immunogen chloramphenicol can effectively prepare the immunogen chloramphenicol and has simple operation process and high production efficiency. The preparation method lays a firm foundation for the improvement in the accuracy and flexibility of a chloramphenicol medicament residue enzyme-linked immunodetection method. In addition, the preparation method can be popularized, promoted and used easily.

Description

Technical field: [0001] The invention relates to a chloramphenicol immunogen, in particular to a preparation method of the chloramphenicol immunogen. Background technique: [0002] The preparation of chloramphenicol immunogen usually needs to implement the process of synthesizing chloramphenicol immunogen. However, in the process of synthesizing chloramphenicol immunogen, existing methods include mixed anhydride method and diazotization method. The former has complicated operation steps and affects There are many factors, and the use of volatile isobutyl chloroformate is harmful to the human body; the immunogen connecting arm obtained by the latter is too short and the immunogenicity is poor. And, the time spent of existing method needs about 80 hours, like this, can make the prolongation of synthetic chloramphenicol immunogen process, cause production efficiency to reduce, product cost increases, simultaneously, follow-up use cost also along with raising, in addition, its ...

AI Technical Summary

Problems solved by technology

[0002] The preparation of chloramphenicol immunogen usually needs to implement the process of synthesizing chloramphenicol immunogen. However, in the process of synthesizing chloramphenicol immunogen, existing methods include mixed anhydride method and diazotization method. The former has complicated operation steps and affects There are many factors, and the use of volatile isobutyl chloroformate is harmful to the human body; the immunogen linking arm obtained by the latter is too short, and the immunogenicity is poor

And, the time spent of existing method needs about 80 hours, like this, can make the prolongation of synthetic chloramphenicol immunogen process, cause production efficiency to reduce, product cost increases, simultaneously, follow-up use cost also along with raising, in addition, its The coupling ratio is generally only around 4-12