Method for preparing ophiopogan total saponies

A technology of total saponins of Radix Ophiopogon japonicus and Radix Ophiopogon japonicus, which is applied in the field of preparation of total saponins of Ophiopogon japonicus, can solve the problems of long total extraction time, multiple extraction times, and large solvent consumption, and achieve shortening extraction time, improving extraction rate, and solvent usage. less effect

Active Publication Date: 2010-07-21
GUANGZHOU HANFANG PHARMA
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Problems solved by technology

It also shows the disadvantages of many times of extraction and long time. It needs to be extracted for a total of 3 hours.
[0006] Based on the above-mentioned documents, it is found that the method used is extracted substantially completely, but there are many solvent consumption, many extraction times, and long shortcoming of total extraction time. In order to save solvent and energy consumption and shorten the extraction time, it is necessary to improve the total extraction time of Radix Ophiopogon japonicus. Extraction method of saponin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The selection of embodiment 1 extraction process condition

[0027] (1) Effect of ultrasonic time on the extraction effect of total saponins of Ophiopogon japonicus

[0028] Experimental method: Take 500g of Ophiopogon japonicus powder that has been crushed to 10-20 meshes, put it in an ultrasonic extractor, add 80% ethanol as the extraction solvent, the weight-to-volume ratio of the medicinal material to the solvent is 1:10, the ultrasonic frequency is 40kHz, and the extraction temperature is 70 ℃, choose different ultrasonic time to extract once, the extract after ultrasonic extraction is filtered or centrifuged, the supernatant is concentrated, extracted with n-butanol to prepare a thick paste, and dried to obtain the Ophiopogon japonicus extract. The extraction effect of total saponins of Ophiopogon japonicus under different extraction time was investigated. The results are shown in Table 1:

[0029] Table 1

[0030] ...

Embodiment 2

[0090] Get 500g of Ophiopogon japonicus medicinal material powder that has been crushed through a 40 mesh sieve, place it in an ultrasonic extractor, the ethanol concentration is 80%, the weight-to-volume ratio of the medicinal material and solvent is 1:20, the ultrasonic frequency is 40kHz, the extraction temperature is 70°C, and the extraction time is 90min , the extract after ultrasonic extraction is filtered or centrifuged, the supernatant is concentrated, extracted with n-butanol to prepare a thick paste, and dried to obtain the total saponins of Ophiopogon japonicus. Results: The dry paste yield was 2.0%, the content was 15.0%, and the extraction rate was 99.3%.

Embodiment 3

[0092] As shown in Example 2, the difference is that the weight-to-volume ratio of the medicinal material to the solvent in Example 2 is 1:20, while the weight-to-volume ratio of the medicinal material to the solvent in Example 3 is 1:10. Results: The yield of dry cream was 1.8%, the content was 15.4%, and the extraction rate was 91.8%.

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Abstract

The invention discloses a method for preparing ophiopogan total saponies by adopting ultrasonic wave enhanced extraction. The method comprises the following steps: grinding the ophiopogan medical material into powder with a certain size; placing the powder in an ultrasonic extractor, wherein, water or alcoholic solution is taken as a solvent, and the weight volume ratio of the ophiopogon japonicus to the solvent is 1:5-1:40; extracting the mixture under supersonic frequency of 20-150kHz and temperature of 20-80 DEG C for 20-200min, and discharging; filtrating, concentrating clear liquid, and drying thick paste after n-butyl alcohol extraction, thus obtaining the ophiopogan total saponies. The preparation method of the invention has the advantages of short extraction time, less times, low temperature, high efficiency and the like, which is applicable for the requirements of industrialized batch production.

Description

technical field [0001] The invention relates to the extraction technology of Ophiopogon japonicus, in particular to a preparation method of Ophiopogon japonicus total saponins. Background technique [0002] Ophiopogon japonicus (L.f.) ker-Gawl is the dried tuber root of Liliaceae Ophiopogon japonicus (L.f.) ker-Gawl. It is a commonly used traditional Chinese medicine for nourishing yin. There are about 40 kinds of medicinal preparations containing Ophiopogon japonicus in the 2005 edition of Chinese Pharmacopoeia. . Ophiopogon japonicus has the functions of nourishing yin and promoting body fluid, nourishing the lungs and clearing the heart, and can be used for symptoms such as febrile disease, body fluid injury, upset and thirst. Modern research has found that Ophiopogon japonicus contains steroidal saponins, high isoflavones, polysaccharides, amino acids and other ingredients. Saponins are one of its active ingredients, which can improve myocardial contractility, protect ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8968A61P9/10A61K125/00
Inventor 蔡鸿飞李金华周珊葛发欢周锦珂黄裕
Owner GUANGZHOU HANFANG PHARMA
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