Preparation method of grape seed procyanidin
A technology of proanthocyanidins and grape seeds, applied in the direction of organic chemistry and the like, can solve the problems of high production cost and large amount of organic solvent used, and achieve the effects of reducing the extraction cost, reducing the amount of organic solvent and improving the extraction efficiency.
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Embodiment 1
[0019] 1. Take 100 grams of grape seeds, add 300ml of water and heat to 85°C, extract with hot water solution for 10 hours, filter to remove grape seeds, and collect the extract.
[0020] 2. Heat the above extract to 100°C, evaporate the water, and concentrate the solution to 30ml.
[0021] 3. Add 60ml of 95% ethanol to the concentrated solution, and stir gently for 2 minutes to dissolve the proanthocyanidins.
[0022] 4. Filter the above solution, collect the filtrate, and discard the insoluble matter.
[0023] 5. Adsorb and separate the proanthocyanidins from the filtrate with a chromatographic column, then elute with 30% ethanol aqueous solution, collect the eluate and dry it to obtain about 1.5 grams of proanthocyanidin products.
Embodiment 2
[0025] 1. Take 100 grams of grape seeds, add 200ml of water and heat to 95°C, extract with hot water solution for 12 hours, filter to remove grape seeds, and collect the extract.
[0026] 2. Heat the above extract to 96°C, evaporate the water, and concentrate the solution to 40ml.
[0027] 3. Add 120ml of 95% ethanol to the concentrated solution, and stir gently for 3 minutes to dissolve the proanthocyanidins.
[0028] 4. Filter the above solution, collect the filtrate, and discard the insoluble matter.
[0029] 5. Adsorb and separate the proanthocyanidins from the filtrate with a chromatographic column, then elute with 40% ethanol aqueous solution, collect the eluate and dry it to obtain about 1.5 grams of proanthocyanidin products.
Embodiment 3
[0031] 1. Take 100 grams of grape seeds, add 250ml of water and heat to 90°C, extract with hot water solution for 11 hours, filter to remove grape seeds, and collect the extract.
[0032] 2. Heat the above extract to 98°C, evaporate the water, and concentrate the solution to 35ml.
[0033] 3. Add 90ml of 95% ethanol to the concentrated solution, and stir gently for 2.5 minutes to dissolve the proanthocyanidins.
[0034] 4. Filter the above solution, collect the filtrate, and discard the insoluble matter.
[0035] 5. Adsorb and separate the proanthocyanidins from the filtrate with a chromatographic column, then elute with 35% ethanol aqueous solution, collect the eluate and dry it to obtain about 1.5 grams of proanthocyanidin products.
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