Preparation method of grape seed procyanidin

A technology of proanthocyanidins and grape seeds, applied in the direction of organic chemistry and the like, can solve the problems of high production cost and large amount of organic solvent used, and achieve the effects of reducing the extraction cost, reducing the amount of organic solvent and improving the extraction efficiency.

Active Publication Date: 2013-10-09
QINGDAO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the deficiencies in the prior art, the technical problem to be solved by the present invention is to provide a method for preparing grape seed proanthocyanidins to improve t...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Take 100 grams of grape seeds, add 300ml of water and heat to 85°C, extract with hot water solution for 10 hours, filter to remove grape seeds, and collect the extract.

[0020] 2. Heat the above extract to 100°C, evaporate the water, and concentrate the solution to 30ml.

[0021] 3. Add 60ml of 95% ethanol to the concentrated solution, and stir gently for 2 minutes to dissolve the proanthocyanidins.

[0022] 4. Filter the above solution, collect the filtrate, and discard the insoluble matter.

[0023] 5. Adsorb and separate the proanthocyanidins from the filtrate with a chromatographic column, then elute with 30% ethanol aqueous solution, collect the eluate and dry it to obtain about 1.5 grams of proanthocyanidin products.

Embodiment 2

[0025] 1. Take 100 grams of grape seeds, add 200ml of water and heat to 95°C, extract with hot water solution for 12 hours, filter to remove grape seeds, and collect the extract.

[0026] 2. Heat the above extract to 96°C, evaporate the water, and concentrate the solution to 40ml.

[0027] 3. Add 120ml of 95% ethanol to the concentrated solution, and stir gently for 3 minutes to dissolve the proanthocyanidins.

[0028] 4. Filter the above solution, collect the filtrate, and discard the insoluble matter.

[0029] 5. Adsorb and separate the proanthocyanidins from the filtrate with a chromatographic column, then elute with 40% ethanol aqueous solution, collect the eluate and dry it to obtain about 1.5 grams of proanthocyanidin products.

Embodiment 3

[0031] 1. Take 100 grams of grape seeds, add 250ml of water and heat to 90°C, extract with hot water solution for 11 hours, filter to remove grape seeds, and collect the extract.

[0032] 2. Heat the above extract to 98°C, evaporate the water, and concentrate the solution to 35ml.

[0033] 3. Add 90ml of 95% ethanol to the concentrated solution, and stir gently for 2.5 minutes to dissolve the proanthocyanidins.

[0034] 4. Filter the above solution, collect the filtrate, and discard the insoluble matter.

[0035] 5. Adsorb and separate the proanthocyanidins from the filtrate with a chromatographic column, then elute with 35% ethanol aqueous solution, collect the eluate and dry it to obtain about 1.5 grams of proanthocyanidin products.

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Abstract

The invention discloses a preparation method of grape seed procyanidin, which comprises the following steps: the grape seeds are extracted in the water solution with the temperature of 85 to 95 DEG C, and the grape seed procyanidin crude extract solution is obtained through filtering; then the crude extract solution is heated, evaporated and concentrated, ethanol is added into the concentrated solution to dissolve the procyanidin and to remove the precipitates so as to obtain the grape seed procyanidin ethanol extract solution; and then the ethanol extract solution is undertaken the adsorption and separation with column chromatography, 30 to 40 percent of ethanol water solution is washed out, the elution liquid is collected, and the elution liquid is dried to obtain the oligomer procyanidin product. The method can improve the efficiency and can reduce the consumption of the solvent and the extraction cost.

Description

technical field [0001] The invention relates to a method for preparing proanthocyanidins in grape seeds, in particular to a method for extracting proanthocyanidins from grape seeds in a high-temperature aqueous solution, which belongs to the field of extracting natural active substances in biotechnology. Background technique [0002] Grape seed procyanidine (grape seed procyanidine) is a natural plant active substance present in grape seeds. It has good antioxidant and free radical scavenging effects, and has many applications in food, health care products, medicines and other fields. The basic structure of grape seed proanthocyanidins is a polymer formed by the polymerization of different amounts of catechin or epicatechin monomers. Usually, those containing 2-4 monomers are called oligomersic procyanidines, which contain More than 4 monomers are called high polymer proanthocyanidins (polymersic procyanidine). At present, proanthocyanidin products sold and applied in the d...

Claims

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Application Information

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IPC IPC(8): C07D311/62
Inventor 孙建平钟进义王可平
Owner QINGDAO UNIV
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