New compositions and methods for cell killing
A cell, target cell technology, applied in the fields of botanical equipment and methods, chemicals for biological control, biocides, etc.
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Embodiment 1
[0117] Cytotoxic effect of polyacrylamide gel (PAAG)-coated and uncoated silica beads on Jurkat cells
[0118] Materials and methods
[0119] Uncoated silica beads (~40 nm in size, Sigma, Cat. No. 421553) were in suspension and coated by photopolymerization with polyacrylamide to introduce acidic and basic acrylamide derivatives Silica beads (immobilized electrolyte) were stored in a +4°C freezer until use.
[0120] The acute T cell leukemia Jurkat cell line - clone E6-1 (ATCC number TIB-152) was used. Jurkat cells were maintained in RPMI-1640 medium supplemented with 1 mmol sodium pyruvate, 10% FBS, and penicillin-streptomycin-amphotericin (1:100).
[0121] Viability and microscopy observations
[0122] Add 2 μl of beads (diluted with 0.1% SDS solution) to 10 in 25 μl of PBS 6 in Jurkat cells. LIVE / DEAD dye (LIVE-DEAD viability kit, Molecular Probes) (0.15 μl) was added and incubated at room temperature. Cell morphology and viability were examined using a fluorescen...
Embodiment 2
[0133] Cytotoxic effect of PAAG beads on Jurkat cells
[0134] Materials and methods
[0135] PAAG beads (~500 nm in size) incorporating immobilized electrolyte (immobiline) at various pHs were prepared using standard emulsification techniques. Store the stock solution in a +4°C refrigerator until use.
[0136] The acute T-cell leukemia Jurkat cell line - clone E6-1 (ATCC accession number TIB-152) was used. Jurkat cells were maintained in RPMI-1640 medium supplemented with 1 mmol sodium pyruvate, 10% FBS, and penicillin-streptomycin-amphotericin (1:100).
[0137] Viability and microscopy observations
[0138] Add 2 μl of beads (diluted with 0.1% SDS solution) to 10 in 25 μl of PBS 6in Jurkat cells. Add LIVE / DEAD dye (LIVE-DEAD viability kit, Molecular Probes) (0.15 μl) and incubate at room temperature. Cell morphology and viability were examined using a fluorescence microscope (Axioskop2plus; filters 4-3).
[0139] result
[0140] Microscopy of Jurkat cells treat...
Embodiment 3
[0146] Cytotoxic effect of two Amberlite TM beads CG-120-I and CG-400-II on Jurkat cells
[0147] Materials and methods
[0148] The cytotoxic effect of two Amberlite TM beads CG-120-I and CG-400-II on Jurkat cells was tested. Among them, Amberlite TM CG-120-II (Fluka, 06469) is sulfonic acid functionalized, Na + Form, 200~400 mesh strong acid gel type resin; AmberliteTM CG-400-II (Fluka, 06471) is quaternary ammonium functionalized, Cl - Form, 200-400 mesh strong basic gel-type resin.
[0149] Add 0.15 μl of dye mix (LIVE / DEAD viability kit from Molecular Probes) to 20 μl of Jurkat cells (5 × 10 5 cells). Amberlite TM beads (5 × 10 5 beads) were added to the cell suspension. Immediately transfer 7 μl of the stained cell suspension onto a microscope slide and cover slip. Live and dead Jurkat cells were measured under a fluorescence microscope using a 4-3 green filter.
[0150] result
[0151] The results showed no substantial difference between the control and the...
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