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A kind of method of preparing phosphate oligosaccharide by whole enzyme method

A technology for oligosaccharide phosphate and enzymatic preparation, applied in fermentation and other directions, can solve problems such as meticulous research on the production of unphosphated oligosaccharides, and achieve the effects of less energy consumption, large resin exchange capacity, and obvious desalination effect.

Inactive Publication Date: 2012-02-01
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

In the past ten years, domestic research and reports on oligophosphate oligosaccharides have been few, and there are only simple reports on oligophosphate oligosaccharide precursors and detection methods, and no detailed research has been done on the production of phosphate oligosaccharides

Method used

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  • A kind of method of preparing phosphate oligosaccharide by whole enzyme method
  • A kind of method of preparing phosphate oligosaccharide by whole enzyme method
  • A kind of method of preparing phosphate oligosaccharide by whole enzyme method

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Embodiment Construction

[0018] 1. Specific operation steps:

[0019] 1) Blending. First pour water into the batching tank, then slowly add 1 ton of raw starch under constant stirring, and use a Bomei meter for online detection until the slurry concentration is 10 o Be, then add 0.65kg CaCl 2 As an enzyme activity accelerator, use HCl and Na 2 CO 3 Adjust the slurry to a pH of 5.4.

[0020] 2) Liquefaction. After pumping the material into the jet liquefier, add a new type of high-temperature-resistant α-amylase to it, with an amount of 2,100,000u / ton of starch. In the jet, the slurry and the steam meet directly and fully, and the jet temperature is 110°C, and maintained for 4 ~8min, control the discharge temperature at 95~97°C, spray the liquefied feed liquid into the laminar flow tank, keep it at 95°C for 30min, and when the typical test reaction shows the true color of iodine, pass steam to kill the enzyme. The DE value of the liquefied material is 15% to 17%.

[0021] 3) Primary plate and fr...

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Abstract

The invention discloses a method for preparing functional oligosaccharide-phosphate oligosaccharide by using potato starch as a raw material and adopting an all-enzyme method. Potato starch is liquefied by using a new type of high temperature resistant α-amylase combined with low-pressure steam jet liquefaction technology, and CaCl2 is added as an enzyme activity accelerator. The synergistic action of pullulanase makes the conversion rate of starch more than 98%, and prepares maltooligosaccharide syrup with low DE value. Use 001×7 strongly acidic styrene-based cation-exchange resin to treat the syrup to remove some positively charged impurities and pigment substances, and then use 7170 strongly-basic styrene-based anion-exchange resin to separate and remove the neutral sugar to obtain Negatively charged phosphate oligosaccharides. Finally, the separated sugar liquid is concentrated and dried, which is the target product.

Description

1. Technical field [0001] The invention relates to a preparation method of oligosaccharides, specifically a method for preparing a novel functional oligosaccharide-phosphate oligosaccharide by using potato starch as a raw material and adopting an all-enzyme method. 2. Technical background [0002] Oligosaccharides, also known as oligosaccharides, refer to linear or branched low-level polymers formed by connecting 2 to 10 monosaccharides through glycosidic bonds. According to the biological functions of oligosaccharides, they can be divided into two categories: functional oligosaccharides and ordinary oligosaccharides. Functional oligosaccharides refer to those with special physiological functions, especially those that are not absorbed by the intestinal tract of humans and animals and promote biologic function. Proliferation of fidobacteria, a class of oligosaccharides beneficial to gut health, the bifidofactors. [0003] The concept of phosphate oligosaccharides (Phosphory...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/00C12P19/14
Inventor 杜先锋杨文军刘霞
Owner ANHUI AGRICULTURAL UNIVERSITY
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